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関連する概念動画

Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

20.2K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
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Reporter Genes02:11

Reporter Genes

11.4K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.4K
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

1.4K
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
1.4K
Regulated mRNA Transport02:22

Regulated mRNA Transport

5.7K
In eukaryotes, transcription and translation are compartmentalized; an mRNA is first synthesized in the nucleus and then selectively transported to the cytoplasm for protein synthesis. Before transport, a pre-mRNA undergoes several steps of post-transcriptional modifications including splicing, 5' capping, and the addition of a poly-adenine tail. Various proteins bind to the pre-mRNA during these modifications. The mRNA transport takes place with the help of multiple proteins playing...
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What is Gene Expression?01:36

What is Gene Expression?

10.1K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
10.1K
What is Gene Expression?01:42

What is Gene Expression?

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Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
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関連する実験動画

Updated: May 1, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

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ローカル・トランスレーションによる遺伝子機能の遠隔制御

Hosung Jung1, Christos G Gkogkas2, Nahum Sonenberg3

  • 1Department of Anatomy, Brain Research Institute, and Brain Korea 21 PLUS Project for Medical Science, Yonsei University College of Medicine, Seoul 120-752, South Korea.

Cell
|April 1, 2014
PubMed
まとめ

RNAの局所化は,タンパク質合成を特定の細胞部位に誘導し,細胞機能にとって極めて重要です. このプロセスは,特に神経細胞において不可欠であり,適切なタンパク質の局所化とホメオスタシスを確保します.

さらに関連する動画

Quantitative Immunofluorescence to Measure Global Localized Translation
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Quantitative Immunofluorescence to Measure Global Localized Translation

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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation

Published on: April 30, 2014

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関連する実験動画

Last Updated: May 1, 2026

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
08:54

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

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Quantitative Immunofluorescence to Measure Global Localized Translation
09:13

Quantitative Immunofluorescence to Measure Global Localized Translation

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In vivo Interrogation of Central Nervous System Translatome by Polyribosome Fractionation
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科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 神経科学は神経科学である.

背景:

  • タンパク質の機能は,その細胞下の位置と密接に結びついている.
  • RNAの局所化は,タンパク質の位置を制御する進化的に保存されたメカニズムです.
  • 局所的なmRNA翻訳は,オンサイトタンパク質合成を可能にし,細胞シグナル伝達とプロテオームバランスに影響を与えます.

研究 の 目的:

  • RNAのローカライゼーションとローカルトランスレーションにおける重要な発見をレビューする.
  • 空間的遺伝子機能制御の意味について議論する.
  • これらのメカニズムがニューロンのコンパートメントにおける重要性を強調する.

主な方法:

  • RNAローカライゼーションとローカルトランスレーションに関する既存の文献のレビュー.
  • 局所的なmRNAとタンパク質合成の役割を実証する研究の分析.
  • 実験的証拠と理論的含意についての議論.

主要な成果:

  • RNAの局所化は,タンパク質が特定のサブセルラー部位で合成されることを保証します.
  • ローカル・トランスレーションは,ユニークなシグナル伝達特性を与え,タンパク質ホメオスタシスを維持します.
  • RNAの局所化と翻訳の調節不良は,神経細胞の欠陥につながる.

結論:

  • RNAの局所化と局所翻訳は,遺伝子機能の空間的制御に不可欠である.
  • これらのメカニズムは,神経細胞のディスタル部位において特に重要である.
  • これらのプロセスを理解することで,神経細胞の発達,配線,生存に関する洞察が得られます.