ファイブロブラストミトーシス中にリン酸化された部位における浄化された成熟促進因子リン酸化物 pp60c-src
S Shenoy1, J K Choi, S Bagrodia
1Department of Molecular and Cell Biology, Pennsylvania State University, University Park 16802.
Cell
|June 2, 1989
まとめ
鶏 pp60c-srcは,成熟促進因子 (MPF) によって特定の部位で新しいミトーシスリン酸化を経験します. この変化したリン酸化が pp60c-src を説明する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- 過剰発現したチキン pp60c-srcは,NIH 3T3細胞ミトーシス中に変化した運動性とリン酸化を示します.
- ミトスリン酸化は,細胞のプロセスとタンパク質の機能の調節に不可欠です.
研究 の 目的:
- pp60c-src.に新しいミトスリン酸化部位を特定する.
- ミトーシス中のpp60c-srcリン酸化における成熟促進因子 (MPF) の役割を調査する.
- 変化したリン酸化が,ppp60c-srcの移動性における観察された変化を説明するかどうかを判断する.
主な方法:
- pp60c-src.のサイト指向型変異およびリン酸化分析.
- Xenopusの卵から精製されたMPFを用いたインビトロリン酸化アッセイ.
- フォスフォリレーションの影響を評価するために,電泳性移動シフトアッセイ.
主要な成果:
- pp60c-src上のThr34,Thr46およびSer72で新規のミトシスリン酸化が特定されました.
- これらのサイトは,p34cdc2.2.の触媒サブユニットであるMPFによって認識されます.
- これらの部位でのpp60c-srcのMPF媒介のリン酸化は,電泳運動の遅延を観察するのに十分である.
- ミトスのpp60c-srcの脱酸化により,正常な運動能力が回復した.
結論:
- pp60c-srcはミトーシス中のMPFの直接標的である.
- pp60c-srcのMPF誘発のリン酸化は,ミトーシス中に観察される細胞の変化に寄与する.
- これらの発見は,pp60c-src活動の調節とミトスの進行におけるその役割についての洞察を提供します.
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