cdc2の可逆タイロシンリン酸化:脱リン酸化は,ミトーシスへの入り口の際に活性化に伴います
A O Morla1, G Draetta, D Beach
1Department of Biology, University of California, La Jolla, San Diego 92093.
Cell
|July 14, 1989
まとめ
細胞循環の調節には,cdc2キナーゼのチロシンリン酸化が含まれています. cdc2の脱酸化はミトスの活性化に不可欠であり,細胞サイクル進行におけるその役割を強調しています.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- Cdc2キナーゼの活動は,細胞サイクル中に厳格に調節されています.
- ティロシンリン酸化は,cdc2の活性を調節する役割を果たします.
研究 の 目的:
- マウス3T3線維芽細胞の細胞サイクルにおけるcdc2のチロシン酸化と脱酸化の役割を調査する.
- cdc2のリン酸化状態とそのキナーゼ活性との関係を決定する.
主な方法:
- 異なる細胞サイクル段階におけるcdc2チロシンリン酸化レベルの分析.
- cdc2.2.のヒストンH1キナーゼ活性に対する測定
- フォスファターゼ阻害剤を使用したチロシン脱フォスホルリレーションの抑制.
主要な成果:
- cdc2のチロシンリン酸化は,G1からG2の段階で増加し,ミトーシス中に減少します.
- チロシンリン酸化cdc2はヒストンH1キナーゼとして無活性である.
- タイロシン脱酸化の抑制は,G2細胞サイクル停止につながる.
結論:
- cdc2のチロシン脱酸化は,ミトーシス中の活性化に不可欠なステップです.
- Cdc2の調節には,細胞サイクル全体を通してチロシンリン酸化状態の動的変化が含まれます.
関連する概念動画
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02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
02:15M-Cdk Drives Transition Into Mitosis
Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...
00:50Anaphase Promoting Complex
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...