Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

7.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
7.5K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

10.2K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
10.2K
Inheritance of Chromatin Structures03:17

Inheritance of Chromatin Structures

6.0K
Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...
6.0K
Lineage Commitment01:21

Lineage Commitment

3.4K
Commitment is the  process whereby stem cells:
3.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Digital health interventions for HIV-related sexual risk behaviors: a systematic review and network meta-analysis.

NPJ digital medicine·2026
Same author

Privacy-Enhanced Vertical Federated Learning for Healthcare via Directional Noise and Subset Representations.

IEEE journal of biomedical and health informatics·2026
Same author

Selection of proper artificial intelligence techniques developed for CT scan image analysis of liver cancer using fuzzy AHP-TOPSIS.

BMC medical imaging·2026
Same author

Identification and functional characterization of a novel mutation in the NEUROD1 gene in a Chinese family with maturity-onset diabetes of the young.

Acta diabetologica·2026
Same author

MFDA-UNet: Medical Image Segmentation with Frequency-Decoupled Representation and Gated Cross-Scale Integration.

Sensors (Basel, Switzerland)·2026
Same author

Analysis of Long-Term and Short-Term Efficacy of Different Types of Tympanosclerosis Surgery Under Total Otoendoscopy.

The Annals of otology, rhinology, and laryngology·2026

関連する実験動画

Updated: May 1, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.0K

STAT3結合の長いノンコーディングRNA lnc-DCは,ヒトの dendritic 細胞の微分化を制御しています.

Pin Wang1, Yiquan Xue, Yanmei Han

  • 1National Key Laboratory of Medical Immunology and Institute of Immunology, Second Military Medical University, Shanghai 200433, China.

Science (New York, N.Y.)
|April 19, 2014
PubMed
まとめ

研究者らは,新しい長いノンコーディングRNA (lncRNA) を発見し,lnc-DCと名付け, dendritic cell (DC) の分化と機能に不可欠である. このlncRNAはSTAT3を活性化させ,T細胞の活性化と免疫反応を高めます.

さらに関連する動画

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

7.6K
A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
11:34

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes

Published on: April 11, 2025

936

関連する実験動画

Last Updated: May 1, 2026

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.0K
Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

7.6K
A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes
11:34

A Simple and Efficient Method for Testing Immunomodulatory Agents for Generation of Tolerogenic Dendritic Cells from Human CD14+ Monocytes

Published on: April 11, 2025

936

科学分野:

  • 免疫学 免疫学とは
  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは

背景:

  • 長いノンコーディングRNA (lncRNAs) は,生物学的プロセスにおける役割としてますます認識されています.
  • しかし,免疫細胞の分化と活性におけるそれらの特定の機能は,ほとんど未知のままである.
  • lncRNAの関与を理解することは,複雑な免疫調節の解読の鍵です.

研究 の 目的:

  • 従来の樹状細胞 (DC) の分化と機能に関与する新しいlncRNAを特定し,特徴づけること.
  • 特定されたlncRNAsがDC活動を調節する分子メカニズムを解明する.
  • 免疫細胞の反応の調節体としてのlncRNAsの可能性を調査する.

主な方法:

  • lnc-DCの識別は,人間の従来のDCにのみ適用されます.
  • マウスの骨髄細胞を用いたヒトモノサイトにおけるin vitroノックダウン実験とin vivoの研究.
  • T細胞活性化アッセイの分析.
  • STAT3 (信号トランスデューサーおよびトランスクリプションのアクティベーター3) の活性化経路の調査,タンパク質結合およびリン酸化測定を含む.

主要な成果:

  • lnc-DCは,ヒトの従来のDCで特異的に発現する新しいlncRNAとして特定されました.
  • lnc-DCのノックダウンにより,DCの分化が著しく低下し,T細胞の活性化を刺激する能力が低下しました.
  • lnc-DCは,細胞質のSTAT3に直接結合し,そのリン酸化を促進し,SHP1による脱リン酸化を防止し,STAT3シグナリングを活性化します.

結論:

  • lnc-DCは, dendritic 細胞の分化と機能の重要なレギュラーです.
  • この研究では,STAT3シグナリングの調節を含むlncRNA作用の新しいメカニズムが明らかになりました.
  • この発見は,免疫におけるlncRNAsの既知の役割を拡大し,免疫細胞の調節に関する新しい洞察を提供します.