関連する実験動画
Updated: Jul 12, 2026

11:44
A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3
Published on: January 24, 2016
構造的には似ているが,機能的には異なる因子であるIRF-1とIRF-2は,IFNとIFN誘導性遺伝子の同じ規制要素と結合する
H Harada1, T Fujita, M Miyamoto
1Institute for Molecular and Cellular Biology, Osaka University, Japan.
Cell
|August 25, 1989
まとめ
研究者らは,IRF-2とIRF-1と同じDNA配列と相互作用する新しいタンパク質であるIRF-2を発見しました. IRF-2は抑制剤として作用し,IRF-1と対抗する.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- ウイルス感染症はI型インターフェロン (IFN) 遺伝子発現を誘発する.
- このプロセスは,IRF-1のような因子によって,規制DNA要素に結合するトランスクリプションの活性化を含みます.
- IFN遺伝子誘導は,典型的には一時的なものです.
研究 の 目的:
- インターフェロン遺伝子調節に関与する新しい要因を特定し,特徴づけること.
- 新しく発見された要因と既知の規制要素の相互作用を理解する.
- 遺伝子転写におけるこれらの因子の相反する役割を解明する.
主な方法:
- 新型因子をコードするcDNAを分離し,IRF-2と指定した.
- IRF-2がIRF-1によって認識されたDNA配列との相互作用の分析.
- IRF-2が遺伝子活性化に及ぼす機能的効果を調査し,特にIRF-1に関連して調査した.
主要な成果:
- 新しい因子であるIRF-2が分離され,IRF-1と同じシス作用要素と結合することが判明しました.
- IRF-1とIRF-2の両方の遺伝子発現は,ウイルスとIFNによって誘導されます.
- IRF-2は抑制剤として機能し,特定の条件下でIRF-1の活性化機能を抑制します.
結論:
- インターフェロンおよびIFN誘導性遺伝子転写は,IRF-1およびIRF-2という2つの類似のトランス作用因子によって調節されます.
- これらの要因は,同じDNA認識配列を争う.
- IRF-1とIRF-2は対極的な効果を有しており,免疫反応の微妙な調節メカニズムを示唆しています.
関連する概念動画
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
NF-κB-dependent Signaling Pathway
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...
Regulation of the Unfolded Protein Response
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

