PADGEMタンパク質:活性化された血小板と中性粒子の相互作用を媒介する受容体
E Larsen1, A Celi, G E Gilbert
1Division of Hematology/Oncology, New England Medical Center, Boston, Massachusetts.
Cell
|October 20, 1989
まとめ
血小板活性化依存の粒子外膜タンパク質 (PADGEM) は,中性粒子と単細胞への活性化血小板粘着を媒介する. PADGEMの機能は,他の血管細胞粘着分子と似ています.
科学分野:
- バイオケミストリー バイオケミストリー
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
背景:
- 血小板活性化依存の粒子外膜タンパク質 (PADGEM) は,血小板アルファ粒子および内皮Weibel-Palade体で見つかりました.
- PADGEMは活性化と粒子の放出後に細胞表面に発現する.
研究 の 目的:
- 細胞粘着におけるPADGEMの役割を調査する.
- PADGEMが活性化された血小板の結合を他の細胞タイプに媒介するかどうかを判断する.
主な方法:
- 活性化された血小板が中性子,単細胞,HL60細胞,U937細胞に結合することを研究した.
- この相互作用に対する抗PADGEM抗体,PADGEM,EDTAの抑制効果を調査した.
- PADGEMを含むフォスフォリピドベシクルを使用して,さまざまな細胞タイプにPADGEM認識部位の存在を評価しました.
主要な成果:
- 活性化された血小板は中性子や単細胞と結合するが,休息している血小板は結合しない.
- この相互作用は,抗PADGEM抗体,PADGEM,EDTAによって特異的に抑制されます.
- 中性粒子の細胞とU937細胞にはPADGEMの認識部位があり,PADGEMがこれらの細胞への血小板の結合を媒介することを示しています.
結論:
- PADGEMは,単細胞と中性粒子の活性化された血小板粘着の重要な媒介です.
- PADGEMは,ELAM-1とMEL-14との機能的および構造的な類似性を示しており,血管細胞粘着分子の新しいファミリーに属することを示唆しています.
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