翻訳開始部位で濃縮された一時停止シーケンスが,体内での転写ダイナミクスを駆動する

Matthew H Larson¹, Rachel A Mooney², Jason M Peters³

¹Department of Cellular and Molecular Pharmacology, Howard Hughes Medical Institute, California Institute for Quantitative Biosciences, Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA.

Science (New York, N.Y.)

|May 3, 2014

まとめ

研究者らは,RNAポリメラーゼ (RNAP) が細菌の転写中に一時停止する16核酸塩基配列を発見した. この発見は,既知の規制停止を説明し,何千もの新しい停止を特定し,保存された停止メカニズムを明らかにします.

科学分野:

微生物学微生物学とは
分子生物学は分子生物学である.
ゲノミクスゲノミクスとは

背景:

バクテリアの転写はRNAポリメラーゼ (RNAP) の休止によって調節されます.
以前の研究は,in vitroの休止に焦点を当て,in vivoの決定因子とゲノム分布が不明のままでした.

研究の目的:

RNAPの配列決定因子を in vivoで特定するために.
これらの一時停止の分布を細菌のゲノム全体にマッピングする.

主な方法:

エシェリキア大腸菌における新生トランスクリプトシーケンシング.
RNAP-核酸相互作用のインビトロ単分子およびアンサンブル分析.

主要な成果:

16ヌクレオチドのコンセンサスパウズシーケンスがE. coliで特定されました.
この配列は,既知の調節停止を説明し,約2万の新しい in vivo 停止部位を特定します.
コンセンサス配列は,RNAP-核酸相互作用によってニュクレオチド添加を阻害し,バクテリア系全体にわたって保存され,一時停止を引き起こします.

結論:

保存されたシーケンスメカニズムは,既知のおよび新しいRNAPの一時停止イベントの両方をベースにしています.
特定されたパウズ配列は,E. coliとBacillus subtilis.の翻訳開始部位で濃縮されています.
この発見は,細菌における転写停止の統一的な理解を提供します.

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