量調節アニオンチャネルVRACの重要な構成要素としてLRRC8ヘテロメアの識別
Felizia K Voss1, Florian Ullrich, Jonas Münch
1Leibniz-Institut für Molekulare Pharmakologie (FMP), Berlin.
まとめ
研究者らは,LRRC8Aを体積調節アニオンチャネル (VRAC) の重要な構成要素として特定した. この発見は,VRACを説明する.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- 生理学 生理学とは
背景:
- 細胞体積の調節は,細胞と生物の機能に不可欠である.
- 容量調節アニオンチャネル (VRAC) の分子同一性は,以前は知られていなかった.
研究 の 目的:
- VRACの分子成分を特定するために.
- VRACの構造と機能を明らかにする.
主な方法:
- 哺乳類の細胞における全ゲノム規模の小干渉RNA (siRNA) スクリーン.
- LRRC8タンパク質の異体化分析.
- 遺伝子破壊と再構成の実験.
- VRAC電流の電気生理学的記録.
- タウリン流量および調節体積減少の測定.
主要な成果:
- LRRC8Aは,VRAC.の重要な構成要素として特定されました.
- LRRC8Aは,他のLRRC8ファミリーメンバーとヘテロマーを形成します.
- LRRC8Aの障害により,VRAC電流が廃止されました.
- VRACの活性を再構成するには,特定のLRRC8イソフォームの組み合わせが必要であり,不活性化運動に影響を与えます.
- タウリン流量と調節量減少は,LRRC8タンパク質に依存していた.
結論:
- VRACはアニオンチャネルの一種を表しています.
- VRACは,体積に敏感な有機オスモリート/アニオンチャンネル (VSOAC) とほぼ同じである.
- LRRC8遺伝子ファミリーは,ネイティブVRAC電流で観察された異質性を説明します.
さらに関連する動画
12:01Acute Dissociation of Lamprey Reticulospinal Axons to Enable Recording from the Release Face Membrane of Individual Functional Presynaptic Terminals
Published on: October 1, 2014
7.7K
15:29Whole-Cell Recording of Calcium Release-Activated Calcium CRAC Currents in Human T Lymphocytes
Published on: December 21, 2010
15.5K
関連する概念動画
Rab Cascades
2.8K
Rab GTPases act in a regulated cascade during membrane fusion, helping the lipid bilayers mix. The Rab family of proteins are active when bound to GTP, and inactive when bound to GDP. Hence, they act as guanine nucleotide-dependent molecular switches. Rab-GTP recognizes and binds to long or short-range tethering proteins to capture the target vesicle. These tethers coordinate with SNAREs on the vesicle and the target membrane to assemble the trans SNARE complex that locks the mixing bilayers.
2.8K
Rab Proteins
4.0K
Rab proteins constitute the largest family of monomeric GTPases, of which 70 members are present in humans. Rab proteins and their effectors regulate consecutive stages of vesicle transport such as vesicle transport, docking, and fusion to the correct recipient membrane.
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
Rab proteins switch between a cytosolic, GDP-bound inactive state and a membrane-anchored, GTP-bound active state. By themselves, Rabs show slow rates of GDP/GTP exchange and GTP hydrolysis. Thus, Rab proteins are considered...
4.0K
Receptor Downregulation in MVBs
2.0K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
The EGFR can initiate signaling pathways that lead to cell proliferation, migration, and differentiation. Overexpression of EGFR stimulates cells to proliferate. Excessive EGFR...
2.0K
Ligand-gated Ion Channels
11.4K
Ligand-gated ion channels are transmembrane proteins with a channel for ions to pass through and a binding site for a ligand. The channel opens only when a ligand attaches to the binding site.
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
Three Subfamilies of Ligand-gated Ion Channels
Ligand-gated ion channels fall into three subfamilies. The 'Cys-loop' includes the nicotinic acetylcholine receptors, γ-aminobutyric acid (GABA), glycine, and 5-hydroxytryptamine receptors. The second one is the 'Pore-loop' channels that...
11.4K
Ligand-gated Ion Channels
8.6K
8.6K
Ligand-Gated Ion Channel Receptor: Gating Mechanism
4.6K
Ligand-gated ion channels are transmembrane proteins that play a vital role in intercellular communication and functions of the nervous system. They allow the influx of ions across the membrane once the neurotransmitter binds, allowing the subsequent transmission of electrical excitation across the neurons. Other ligand-gated ion channels, like the γ-aminobutyric acid (GABA) receptor, permit anions like chloride into the cells on the binding of the GABA molecule. Their entry into the cell...
4.6K
