2-チオシュガー生物合成のための主要な代謝経路から硫黄媒介タンパク質をコオプティングする
Eita Sasaki1, Xuan Zhang2, He G Sun3
1Department of Chemistry, University of Texas at Austin, Austin, Texas 78712, USA.
Nature
|May 13, 2014
まとめ
科学者は,硫黄原子が自然製品で炭素-硫黄結合を形成する方法を解明しました. 彼らは,抗生物質のバイオシンセシスに不可欠なAmycolatopsis orientalisの柔軟な硫黄供給システムを発見しました.
科学分野:
- バイオケミストリー バイオケミストリー
- 分子生物学は分子生物学である.
- 自然製品合成 自然製品の合成
背景:
- 硫黄は生命にとって不可欠ですが,二次代謝産物への組み込みは十分に理解されていません.
- 主要代謝生物合成は,特定の酵素によって活性化された硫黄媒介タンパク質を使用します.
- 通常,これらの酵素とタンパク質の遺伝子は,一緒にクラスタ化されています.
研究 の 目的:
- 抗生物質BE-7585A.の2-チオシューガー部分の生物合成を調査する.
- 硫黄の組み込みに関与する酵素やメカニズムを特定する.
- アミコラトプシス・オリエンタリス (Amycolatopsis orientalis) の硫黄配送システムを理解するために.
主な方法:
- 推定の2チオグルコース合成酵素 (BexX) の識別.
- Amycolatopsis orientalis.のゲノムシーケンシングについて
- 酵素活性とタンパク質の相互作用をテストするための生化学的測定法.
- タンパク質の構造を決定するX線結晶学.
主要な成果:
- BexXは,2-チオグルコース合成酵素として識別され,ThiGに似ています.
- BE-7585Aクラスタでは,同種の硫黄媒介タンパク質遺伝子が見つかりませんでした.
- A. orientalis の1つの活性化酵素は,複数の硫黄媒介タンパク質を修正することができます.
- X線結晶学により,BexXの選択的なタンパク質認識の構造的基礎が明らかになりました.
結論:
- この研究は,天然のチオ糖形成の最初の完全な特徴付けを提供します.
- アミコラトプシス・オリエンタリスは,主および二次代謝の為に乱交的な硫黄供給システムを利用している.
- この発見は,天然製品の生物合成のために,主代謝の硫黄機構を併用する一般的な戦略を示唆している.
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