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関連する概念動画

Nuclear Protein Sorting01:34

Nuclear Protein Sorting

6.7K
Nuclear protein sorting is the selective trafficking of histones, polymerases, gene regulatory proteins into the nucleus and exporting RNAs and ribosomes to the cytosol. It is a tightly controlled process that regulates gene expression within a cell.
Proteins targeted to the nucleus carry nuclear localization signals or NLS recognized by import receptors in the cytosol. Similarly, proteins with nuclear export signals are recognized by export receptors. Import and export receptors are...
6.7K
Nuclear Localization Signals and Import01:46

Nuclear Localization Signals and Import

8.4K
Proteins targeted to the nucleus carry short stretches of amino acid sequences called the nuclear localization signal or NLS. Classical nuclear localization signals are of two types: monopartite and bipartite NLS. Monopartite classical NLS (cNLS) consists of a single cluster of 4-8 amino acids. Bipartite cNLS consists of two clusters of  2-3 amino acids and a 9-12 residue long proline-rich linker bridging the two clusters. Signal clusters are rich in positively charged amino acids such as...
8.4K
Nuclear Export01:42

Nuclear Export

5.3K
The nucleus restricts several proteins within and allows others to pass. The restricted proteins possess a nuclear retention sequence or NRS, anchoring them to the nuclear lamins and preventing their transport to the cytosol. The non-restricted proteins, after their synthesis, are transported to their site of action, such as the cytosol or other organelles, with the help of nuclear export signals or NES.
NES are of three types- the canonical 10-residue long leucine-rich signal and other...
5.3K
Directionality of Nuclear Transport01:42

Directionality of Nuclear Transport

5.2K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of  Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
5.2K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.5K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.5K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

18.4K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
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関連する実験動画

Updated: Apr 10, 2026

Heterokaryon Technique for Analysis of Cell Type-specific Localization
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Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

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Ankyrinの繰り返しでRanGDPを結合するコードは,核輸入経路を定義します.

Min Lu1, Jaroslav Zak1, Shuo Chen1

  • 1Ludwig Institute for Cancer Research, Nuffield Department of Clinical Medicine, University of Oxford, Oxford, OX3 7DQ, UK.

Cell
|May 27, 2014
PubMed
まとめ

科学者たちは,アンキリンリピートタンパク質 (ARP) の新たな核輸入経路を発見した. 水害性残留を含む特定のコードは,インポルチンとは独立してARPを核に誘導し,メラノーマと転写調節に影響を与えます.

さらに関連する動画

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

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Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

Published on: May 9, 2020

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関連する実験動画

Last Updated: Apr 10, 2026

Heterokaryon Technique for Analysis of Cell Type-specific Localization
09:31

Heterokaryon Technique for Analysis of Cell Type-specific Localization

Published on: March 11, 2011

17.1K
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.6K
Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC
09:15

Monitoring Protein-RNA Interaction Dynamics In Vivo at High Temporal Resolution Using χCRAC

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科学分野:

  • 分子生物学は分子生物学である.
  • 細胞生物学 細胞生物学
  • 遺伝学 遺伝学とは

背景:

  • 核の輸入は,真核細胞の機能にとって極めて重要です.
  • ほとんどの核タンパク質は,インポートイン媒介経路を使用します.
  • インポートイン相互作用が欠けているタンパク質にはインポートイン独立経路が必要である.

研究 の 目的:

  • 一般的な輸入独立の原子力輸入経路を特定する.
  • アンキリンリピートタンパク質 (ARP) の核輸入を規制するコードを定義する.
  • この経路が疾患と転写調節における役割を調査する.

主な方法:

  • アンキリンリピート (AR) 内の特定のアミノ酸コードの識別.
  • 17の異なるARPでコードの実験テスト.
  • 150以上の注釈されたヒトARPの核-細胞質の局所化予測.

主要な成果:

  • 連続したARの位置13にある水嫌性残留物は,RanGDP.に効率的な結合を媒介する.
  • この相互作用は,輸入に依存しない核の参入を容易にする.
  • 特定されたコードは,ARPの局所化を正確に予測し,メラノーマにおけるCDKN2A変異と関連しています.

結論:

  • RanGDP/AR (RaDAR) 経路は,輸入独立の核輸入メカニズムである.
  • この経路は,ARP,特にNF-κB/p53.5の転写レギュレータによって頻繁に利用されます.
  • この発見は,メラノーマの病原性および遺伝子調節を理解するための意味を持つ.