E-カデリンによる機械的フィードバックは,集団的な細胞移動の間に方向感知を促進します.
Danfeng Cai1, Shann-Ching Chen2, Mohit Prasad3
1Department of Biological Chemistry, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA; Molecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, CA 93106-9625, USA.
Cell
|May 27, 2014
まとめ
E-カデリンの細胞結合は,集団的な細胞移動を誘導する. この研究は,E-カデリンが,機械的な緊張とフィードバックループを通して,細胞の動きを制御し,化学療法中に組織の完全性を維持する方法を明らかにしています.
科学分野:
- 細胞生物学 細胞生物学
- 発達生物学 発達生物学とは
- バイオフィジックス 生物物理学
背景:
- E-カデリンは,細胞同士の結合の鍵となる分子である.
- 密度の高い組織を通る集団的な細胞移動におけるその役割は,完全に理解されていません.
研究 の 目的:
- 集団的な細胞移動におけるE-カデリンの機能を in vivoで調査する.
- E-カデリンが化学作用中に細胞の動きを誘導するメカニズムを解明する.
主な方法:
- E-cadherinを横断する機械的張りのインビボセンサーの開発.
- 細胞タイプ固有のRNAi,光活性化Rac,形態動力学的プロファイリングを使用した.
- ドロソフィラの卵巣を集団的な細胞移動のモデルシステムとして採用した.
主要な成果:
- サブストラット (看護細胞) とのE-カデリン媒介の粘着は,Racとアクチンアセンブリとのポジティブなフィードバックループを形成し,細胞の突起と持続的な方向の動きを安定させます.
- 境界細胞間の細胞間粘着は,先導細胞からフォロワー細胞への方向性信号を伝達する.
- 移動性細胞と極性細胞の結合は,クラスターの結束と個々の細胞の極化を維持する.
結論:
- E-カデリンは,集団的な細胞移動と in vivo の化学反応のオーケストレーションに不可欠です.
- 誘導機構の不可欠な構成要素として機能し,集団の移動中に細胞間のコミュニケーションと組織組織を調節する.
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