成長因子活性を持つラミニンの領域
G Panayotou1, P End, M Aumailley
1National Institute for Medical Research, London, England.
Cell
|January 13, 1989
まとめ
ラミニン断片は,表皮成長因子 (EGF) に関連する経路を通じて細胞成長を刺激し,細胞の成長と粘着のための明確な領域を示します.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- バイオケミストリー バイオケミストリー
背景:
- ラミニンは,細胞結合とシグナル伝達に関与する重要な細胞外マトリックスタンパク質です.
- 皮膚表皮成長因子 (EGF) は,細胞増殖を調節する強力なミトゲンです.
研究 の 目的:
- ラミニンおよびその断片のミトゲン性の可能性を調査する.
- ラミニンの成長促進活動とEGFのシグナル伝達経路の関係を探求する.
- 特定のラミニンドメインがミトゲネシスと細胞粘着に責任があるかどうかを判断する.
主な方法:
- EGF受容体とない培養細胞は,ラミニンとその断片を投与した.
- 細胞増殖を評価するために,チミジン組み込みが測定されました.
- EGFの結合測定は,受容体競争を評価するために行われました.
- 細胞結合測定は,ラミニン断片への粘着度を評価するために使用されました 8.
主要な成果:
- EGFのような繰り返しを含んだラミニン断片は,EGFに似たEGF受容体を持つ細胞にチミジンの組み込みを刺激しました.
- これらの活性ラミニン断片は,EGF受容体が欠けている細胞に影響を与えず,EGF結合と競合しませんでした.
- 非活性ラミニン断片 (4および8) はミトゲン効果を示さなかった;断片8は細胞結合を媒介したが,成長促進はしなかった.
結論:
- ラミニンのミトジェニック効果とEGFは,同じ経路のステップを共有しています.
- 異なるラミニンのドメインは,細胞の成長を促進し,細胞の結合/拡散を媒介する責任を負う.
- 細胞増殖におけるラミニンの役割は,EGF受容体依存の経路を通じて媒介されます.
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