新しいDNA結合と二分化モチーフは,免疫グロブリン増強剤結合,非娘性,MyoD,およびmycタンパク質に含まれています
C Murre1, P S McCaw, D Baltimore
1Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 02142.
Cell
|March 10, 1989
まとめ
研究者らは,DNAを結合する2つの新しいcDNAを特定しました. 彼らの二分化とDNA結合能力は極めて重要であり,既知の発達遺伝子に似た,保存されたヘリックス・ループ・ヘリックスモチーフを伴う.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- タンパク質とDNAの相互作用
背景:
- 免疫グロブリンカッパ鎖強化剤は,規制タンパク質によって認識される特定のDNA配列 (カッパE2) を含んでいます.
- 既存の知識は,特定の遺伝子ファミリー (例えば,Drosophila achaete-scute,twist,myc) を発達プロセスとDNA結合と結びつけている.
研究 の 目的:
- 免疫グロブリンカッパ鎖強化剤の調節に関与する新しいcDNAを特定し,特徴づけること.
- 特定されたcDNAの構造的および機能的性質,特にその二分化およびDNA結合機構を調査する.
主な方法:
- 2つの特定のcDNAの分離とクローン.
- 発達に関する既知の遺伝子 (ドロソフィラ無娘,myc,MyoD,achaete-scute,twist) とのシーケンスのホモロジーの分析.
- ホモロゴス領域の構造予測,アンフィパティックヘリックスと保存された水害性残留物に焦点を当てた.
- 識別されたモチーフがダイメリゼーションとDNA結合における役割の実験的検証.
主要な成果:
- 2つのcDNAが分離され,その二元化製品は特にkappa E2 DNA配列に結合する.
- cDNAには,ドロソフィラ無娘種と他の発達遺伝子の家族に類似した同類領域が含まれています.
- この同質領域は,二分化とDNA結合に不可欠な,高度に保存された水嫌性残留物を持つ,潜在的なヘリックス・ループ・ヘリックス構造を示している.
結論:
- 種と遺伝子ファミリーにわたって保存されている新しいモチーフは,タンパク質二分化と特定のDNA結合に不可欠です.
- これらの発見は,免疫グロブリン遺伝子発現とより広範な発達経路を調節する分子機構に関する新しい洞察を提供します.
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The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
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