Fos/Junタンパク質複合体の配列特異的なDNA相互作用には,Fosの2つの機能的に異なる領域が必要です
M Neuberg1, M Schuermann, J B Hunter
1Institut für Molekularbiologie und Tumorforschung, Phillips-Universität Marburg, FRG.
Nature
|April 13, 1989
まとめ
Fos/Jun複合体はDNAを結合して遺伝子転写を調節する. レウシンジッパーと隣接する基本領域を含むFosの特定の領域は,このDNA結合と転写の調節に不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝子規制 遺伝子規制
- タンパク質とDNAの相互作用
背景:
- 細胞およびレトロウイルスのFosタンパク質とAP1/Jun転写因子によって形成されるFos/Jun複合体は,TRE DNA配列に結合する.
- この結合イベントは,遺伝子転写を刺激する上で極めて重要です.
研究 の 目的:
- 機能的な Fos/Jun/TRE 複合体の形成に不可欠な Fos タンパク質内の特定の領域を特定する.
- タンパク質とタンパク質,タンパク質とDNAの相互作用におけるこれらの領域の役割を解明する.
主な方法:
- サイト指向型ミュータゲネシスにより,Fosタンパク質に特定のアミノ酸の置換を導入する.
- フォス/ジュンタンパク質関連性の分析.
- Fos/Jun/TRE複合体の形成とDNA結合活動の評価.
主要な成果:
- Fos/Jun/TRE複合体の形成には,Fosの2つの異なる領域が必要である:Junの結合のためのルシンジッパーと隣接する基本領域.
- 塩基領域におけるアミノ酸置換は,Fos/JunとTREDNAの結合を妨害したが,Fos/Junとタンパク質の結合には影響しなかった.
- Fosの機能的に重要なアミノ酸はJunとGCN4のDNA結合部位と構造的に類似している.
結論:
- レウシンジッパーに隣接するFosの基本領域は,Fos/Jun複合体のDNA結合を媒介する上で重要な役割を果たしています.
- この領域が他のDNA結合ドメインと構造的に類似していることは,転写調節における保存されたメカニズムを強調している.
関連する概念動画
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
RNA Polymerase II Accessory Proteins
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
Protein Complexes with Interchangeable Parts
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order to...
General Transcription Factors
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...


