タンパク質安定剤としてのカルボキシル化されたグルキュロンのポリアミドサハリド
Sarah E Stidham1, Stacy L Chin, Eric L Dane
1Departments of Chemistry and Biomedical Engineering, Boston University , Boston, Massachusetts 02215, United States.
Journal of the American Chemical Society
|June 21, 2014
まとめ
新しい炭水化物ポリマーであるポリアミドサハリド (PAS) が合成され,酸化され,タンパク質の安定性を高めました. これらの改変されたPASは,トリハロースと比較してリゾ酵素に優れた安定性を提供します.
科学分野:
- ポリマー化学のポリマー化学について
- 炭水化物化学 炭水化物の化学
- バイオマテリアル科学 バイオマテリアル科学
背景:
- 自然のポリサッカライドは,構造的な多様性が限られている.
- 合成ポリマーをカスタマイズすることで,正確な特性制御が可能になります.
- ポリサッカライドのイオン化群は,その機能に影響する.
研究 の 目的:
- 制御されたアニオンポリメリゼーションを使用して,新しいエナティオピュアポリアミドサハリド (PAS) を合成する.
- グルコース由来モノメアの酸化により,イオン化可能なカルボキシル酸群を導入する.
- 酸化したPASの二次構造とタンパク質安定化能力を評価する.
主な方法:
- グルコース由来モノメアの制御されたアニオンポリメリゼーション.
- TEMPO媒介による原発アルコールの炭酸酸への酸化.
- 構造分析のための円形二重化スペクトロスコピー.
- 脱水および凍結ストレス下でのリソ酵素安定化アッセイ.
主要な成果:
- α-1,2アミド結合を持つエナンチオプアPASの合成が成功しました.
- 制御された酸化により,カルボキシル酸群を備えたポリマーが得られ,優れた収量が得られた.
- 酸化したPASは,水溶液中の順番の螺旋状二次構造を採用した.
- 酸化されたPASは,トリハロースと比較して,リゾ酵素の安定性が優れていることが示されました.
結論:
- 新しい炭水化物ベースのポリマー (PAS) は,精密に設計することができます.
- 酸化されたPASは,秩序ある構造と強化されたタンパク質の冷凍保護/冷凍保護を示す.
- これらの合成ポリマーは,バイオ保存アプリケーションのための有望なバイオマテリアルを表しています.
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