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ATP synthase or ATPase is among the most conserved proteins found in bacteria, mammals, and plants. This enzyme can catalyze a forward reaction in response to the electrochemical gradient, producing ATP from ADP and inorganic phosphate. ATP synthase can also work in a reverse direction by hydrolyzing ATP and generating an electrochemical gradient. Different forms of ATP synthases have evolved special features to meet the specific demands of the cell. Based on their specific feature, ATP...
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From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028
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モジュール型ポリケチド合成酵素の構造

Somnath Dutta1, Jonathan R Whicher2, Douglas A Hansen3

  • 11] Life Sciences Institute, University of Michigan, Ann Arbor, Michigan 48109, USA [2].

Nature
|June 27, 2014
PubMed
まとめ
この要約は機械生成です。

研究者は,タイプIポリケチド合成酵素 (PKSs) を研究し,基板移転のための新しいアーキテクチャを明らかにしました. この発見は,これらの重要な生物合成酵素の分子機構に関する新しい洞察を提供します.

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科学分野:

  • バイオケミストリー バイオケミストリー
  • 分子生物学は分子生物学である.
  • 構造生物学 構造生物学とは

背景:

  • ポリケチドの天然製品は,多様な構造と生物活性を持っています.
  • I型ポリケチド合成酵素 (PKSs) は,その生物合成を担当する大型モジュール型酵素である.
  • PKSアーキテクチャを理解することは,ポリケチド合成経路の解明の鍵です.

研究 の 目的:

  • *Streptomyces venezuelae*からの全長PKSモジュールの3次元構造を決定する.
  • PKSモジュール内および PKSモジュール間の基板移転のメカニズムを解明する.
  • PKSシステムの構造分析のための新しいモデルを確立する.

主な方法:

  • 電子冷凍顕微鏡 (cryo-EM) を使用して,高解像度の構造データを取得しました.
  • サブナノメートルの解像度の3次元再構築が生成されました.
  • 構造的な比較は,哺乳類の脂肪酸合成酵素などの同種酵素と行われた.

主要な成果:

  • 哺乳類の脂肪酸合成酵素と異なるユニークなPKSモジュール構造が明らかになった.
  • 単一の反応室は,イントラモジュールキャリアドメインを収容します.
  • 独立したエントリーポイントは,イントラモジュール処理とは異なり,インターモジュール基板の配送を容易にします.

結論:

  • この研究は,PKSにおけるイントラモジュールおよびインターモジュール基板移転に関する最初の構造的証拠を提供します.
  • PKS酵素機能と基板チャネリングのための新しいモデルが提案されています.
  • この研究は,複雑なPKS機構の分子解剖のための新しい戦略を可能にします.