Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

Overview
Antigens Involved in Adaptive Immunity01:26

Antigens Involved in Adaptive Immunity

An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and reactivity.
Diversity of Antigen Receptors01:28

Diversity of Antigen Receptors

Antigen receptors are essential components of the immune system crucial in defending the body against foreign invaders. These receptors are present on the surface of B and T cells, enabling them to recognize antigens and mount an appropriate immune response.
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
Antigen Processing Pathways01:31

Antigen Processing Pathways

MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
B Cell Activation and Differentiation01:24

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

A funnel approach to enable analyses of epitope-specific human CD4 T cells specific for influenza and SARS-CoV-2.

mBio·2026
Same author

The regulation of the immune system.

Immunology today·2014
Same author

Light transmission from a randomly rough dielectric diffuser: theoretical and experimental results.

Optics letters·2009
Same author

Comparison of surface scattering between identical, randomly rough metal and dielectric diffusers.

Optics letters·2009
Same author

Hydrogen bond integrity between MHC class II molecules and bound peptide determines the intracellular fate of MHC class II molecules.

Journal of immunology (Baltimore, Md. : 1950)·2001
Same author

Exclusion of CD43 from the immunological synapse is mediated by phosphorylation-regulated relocation of the cytoskeletal adaptor moesin.

Immunity·2001

関連する実験動画

Updated: Jun 19, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

コンポーネントチェーンに対する細胞内競争は,クラスIIのMHC細胞表面現象型を決定する.

A J Sant1, R N Germain

  • 1Laboratory of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.

Cell
|June 2, 1989
PubMed
まとめ

混合イソタイプダイマーは,通常,血液細胞では見られない. しかし,クラスIIのアルファとベータ鎖の非対称合成により,細胞表面での有意な発現につながる可能性があります.

科学分野:

  • 免疫遺伝学 免疫遺伝学とは
  • 分子免疫学 分子免疫学
  • 細胞免疫学 細胞免疫学

背景:

  • 混合同型ジマー (例えば,EアルファAベータ,AアルファEベータ) は,一般的にIa+血液細胞に存在しない.
  • いくつかの混合同型二元は,必要なクラスII遺伝子のみを含むトランスフェクト細胞の表面に発現することができます.

研究 の 目的:

  • 混同同型二次体の差異表現の背後にある理由を調査する.
  • 細胞モデルで,個々のIaダイマーの表面表現を,利用可能なクラスII鎖と比較する.
  • Ia分子発現におけるチェーン競争の役割を理解する.

主な方法:

  • バランスのとれた非対称なクラスIIアルファとベータ鎖の合成をシミュレートするためにL細胞変異モデルを使用しました.
  • 異なる合成条件下における特定のIaダイマー (AアルファAβ,EアルファEβ,EアルファAβ) の表面表現の比較.
  • 感染した細胞内の量化可能なクラスII鎖.

主要な成果:

  • 主要なAアルファAベータとEアルファEベータ種は,組み立てや輸送で3〜5倍の好みを示しています.
  • これらの好みは,通常,バランスのとれた鎖合成の下で混合同型EアルファAベータジマーの発現を妨げます.

さらに関連する動画

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

関連する実験動画

Last Updated: Jun 19, 2026

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation
12:09

Use of Single Chain MHC Technology to Investigate Co-agonism in Human CD8+ T Cell Activation

Published on: February 28, 2019

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses
10:13

Tailoring In Vivo Cytotoxicity Assays to Study Immunodominance in Tumor-specific CD8+ T Cell Responses

Published on: May 6, 2019

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
09:32

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis

Published on: October 15, 2021

  • 非対称な鎖合成は,混合同型ジメルの生物学的に有意な表面表現を可能にします.

    • 結論:

    • チェーンコンペティションとディフェンシャルアセンブリ/トランスポートキネティクスは,イアダイマー表面表現に影響します.
    • バランスの取れた連鎖合成の生理学的条件は,ヘテロディメアよりもホモディメアを好む.
    • 変化した鎖合成比率は,固有の偏好を克服し,混合同型二重体プレゼンテーションを可能にします.