CTF/NF-Iのプロリンに富んだ転写活性化剤は,複製とDNA結合ドメインとは異なる

N Mermod¹, E A O'Neill, T J Kelly

¹Howard Hughes Medical Institute, University of California Berkeley 94720.

Cell

|August 25, 1989

まとめ

人間のCTF/NF-Iタンパク質はDNAを結合し,転写と複製を調節する. N末端領域はDNA結合と複製を処理し,プロリンに富んだC末端領域は転写を活性化する.

科学分野:

分子生物学は分子生物学である.
遺伝子規制遺伝子規制
ウイルス学ウイルス学ウイルス学

背景:

CTF/NF-Iタンパク質は,転写とDNA複製を含む細胞プロセスに不可欠です.
CTF/NF-Iの機能ドメインを理解することは,遺伝子調節機構の解読の鍵です.

研究の目的:

DNA結合,タンパク質二酸化,転写活性化,DNA複製に関与するヒトCTF/NF-Iの機能領域を定義する.
新規のプロリンに富んだC端末活性化領域を特徴付けるために.

主な方法:

E. coliおよびドロソフィラ細胞で発現するcDNA変異体の分析.
DNA認識,タンパク質二分化,アデノウイルス複製,および異質プロモーター (例えば,SV40) を使用した転写活性化のための機能検査.

主要な成果:

CTF-1のN端部分は,サイト固有のDNA認識,タンパク質二分化,アデノウイルス複製を媒介する.
異なったC端末ドメインは,転写活性化のために必要である.
このプロリン残留に富んだC端末ドメインは,DNA結合ドメインと融合すると異質プロモーターを活性化させます.
プロリンに富んだドメインは,酸性モチーフやグルタミンに富んだモチーフとは異なる新種の転写活性化剤のクラスを表しています.

結論:

CTF/NF-Iの機能はモジュラーで,DNA複製と転写活性化を制御するドメインは別々である.
プロリンに富んだC端末ドメインは,転写活性化剤の新種を定義する.
トランスクリプションの活性化には多様なメカニズムが関与しており,CTF/NF-Iは独自の戦略を採用しています.

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