Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

1.4K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.4K
Forced Transdifferentiation01:28

Forced Transdifferentiation

1.5K
Transdifferentiation, also known as lineage reprogramming, was first discovered by Selman and Kafatos in 1974 in silkmoths. They observed that the moths’ cuticle-producing cells transformed into salt-producing cells. Many such cases of natural transdifferentiation occur in organisms. In humans, pancreatic alpha cells can become beta cells. In newts, the loss of the eye’s lens causes the pigmented epithelial cells to transdifferentiate into the lens cells.
Artificial...
1.5K
Determination01:51

Determination

16.3K
During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
16.3K
Cellular Differentiation00:57

Cellular Differentiation

5.7K
How does a complex organism such as a human develop from a single cell? It all starts from a single fertilized egg which gives rise to a vast array of cell types, such as nerve cells, muscle cells, and epithelial cells that characterize the adult? Throughout development and adulthood, cellular differentiation leads cells to assume their final morphology and physiology. Differentiation is the process by which unspecialized cells become specialized to carry out distinct functions.
A zygote is a...
5.7K
iPS Cell Differentiation01:22

iPS Cell Differentiation

2.2K
The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.
2.2K
Lineage Commitment01:21

Lineage Commitment

3.4K
Commitment is the  process whereby stem cells:
3.4K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Human Sex Chromosome Biology in the Genomic Era.

Annual review of genomics and human genetics·2026
Same author

A novel hyperactive <i>BCR::ABL1</i> <sup><i>e6a3</i></sup> variant confers resistance to combined asciminib plus ponatinib therapy.

medRxiv : the preprint server for health sciences·2026
Same author

Efficient Generation of Functional TCRαβ<sup>+</sup> Cytotoxic T Cells from hiPSCs via Small-Molecule Modulation.

bioRxiv : the preprint server for biology·2026
Same author

Transcriptional mechanisms of sex-biased gene expression and their connections to disease-associated variation.

Human molecular genetics·2026
Same author

Soluble Notch agonist enables human ameloblast maturation and enamel-like tissue formation for tooth regeneration.

International journal of oral science·2026
Same author

Bnip3lb-driven mitophagy maintains fate of the embryonic hematopoietic stem cell pool.

Nature communications·2026

関連する実験動画

Updated: Apr 25, 2026

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

15.2K

エンジニアリングされた細胞タイプを解剖し,CellNetを通じて細胞運命を変換することを強化します.

Samantha A Morris1, Patrick Cahan1, Hu Li2

  • 1Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Manton Center for Orphan Disease Research, Howard Hughes Medical Institute, Boston Children's Hospital and Dana Farber Cancer Institute, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Harvard Stem Cell Institute, Cambridge, MA 02138, USA.

Cell
|August 16, 2014
PubMed
まとめ

ネットワーク生物学プラットフォームであるCellNetは,遺伝子調節エラーを特定し,修正することにより,再生医療のためのエンジニアリング細胞開発を改善しています. この技術は細胞の変換を向上させ,エンジニアリングされた細胞の新たな治療の可能性を明らかにします.

さらに関連する動画

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

9.8K
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

5.6K

関連する実験動画

Last Updated: Apr 25, 2026

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells
13:58

Derivation of Adult Human Fibroblasts and their Direct Conversion into Expandable Neural Progenitor Cells

Published on: July 29, 2015

15.2K
Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells
11:17

Efficient Neural Differentiation using Single-Cell Culture of Human Embryonic Stem Cells

Published on: January 18, 2020

9.8K
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

5.6K

科学分野:

  • 細胞再プログラミングと再生医療
  • ネットワーク生物学と遺伝子規制ネットワーク
  • バイオテクノロジーとバイオエンジニアリング

背景:

  • 現在の細胞工学プロトコルは,しばしば標的細胞の特徴を複製できず,再生医療における応用を制限しています.
  • 細胞のアイデンティティと機能をin vitroで正確に再現することは,依然として大きな課題です.
  • 遺伝子規制ネットワークの理解は,細胞工学の成功に不可欠です.

研究 の 目的:

  • エンジニアリング細胞の評価と改善のためのネットワーク生物学プラットフォームであるCellNetを導入する.
  • エンジニアリングされた細胞の異常な遺伝子調節ネットワークを診断するために.
  • 細胞変換を強化するための転写レギュレータを特定する.

主な方法:

  • セルネットプラットフォームの開発とアプリケーション.
  • 細胞のアイデンティティを制御する遺伝子規制ネットワークの分析.
  • 細胞変換プロトコルにおける予測されたレギュレータの実験的検証 (例えば,B細胞からマクロファージ,線維芽細胞から肝細胞).

主要な成果:

  • CellNetは,B細胞からマクロファージへの変換効率を,トランスクリプション的にも,機能的にも,成功裏に改善しました.
  • CellNetは,Cdx2.2によって調節される,誘発性肝細胞 (iHeps) に変換された線維芽細胞における予期せぬ腸内プログラムを特定しました.
  • 誘発性肝細胞は,マウスの結腸に長期にわたる機能的な移植を実証し,内皮の先駆体としての可能性を示した.

結論:

  • CellNetは,直接的な細胞変換を改善し,エンジニアリングされた細胞の信頼性を確保するための貴重なツールです.
  • このプラットフォームは,これまで評価されていない性質と,エンジニアリングされた細胞の潜在的な応用を明らかにすることができます.
  • この研究は,細胞工学を強化し,新しい細胞機能を発見する方法を提供することにより,再生医療の分野を前進させます.