E. crassusの非常に反復性の高い要素の発達的に調整された大量切除
C L Jahn1, M F Krikau, S Shyman
1Department of Biological Sciences, University of Illinois, Chicago 60680.
Cell
|December 22, 1989
まとめ
E. crassus の Tec1 要素は,マイクロ核のゲノムには豊富に存在するが,マクロ核のゲノムには存在しない. マクロ核発達の初期に切除され,染色体外円を形成する.
科学分野:
- * 分子生物学について
- * 遺伝学について
- * 細胞生物学 細胞生物学
背景:
- * E. crassus Tec1 要素は DNA 配列の繰り返しである.
- * 微核ゲノムに大量に存在する.
- * Tec1はマクロ核ゲノムに顕著に欠けています.
研究 の 目的:
- *マクロ核発達の過程におけるE. crassus Tec1元素の行動と運命を調査する.
- * Tec1の切除とゲノム内の局所化の基礎となる分子メカニズムを特徴づける.
主な方法:
- * Tec1.1.の染色体外循環型および統合型の制限マッピング
- * Tec1 terminiと円形のDNA形態のシーケンス分析.
- *マクロ核ゲノム形成中のTec1元素の行動の分析.
主要な成果:
- * Tec1元素は,マクロ核発達の過程で主に染色体外円を形成する.
- *Circular Tec1の形状はBAL31ヌクレアゼに対する耐性を示しており,反転繰り返しの端末での切除を示しています.
- * 配列解析は,円形のTEC1でヘッド・トゥ・ヘッドの反転繰り返しを確認し,マクロ核発達の初期に切除が起こりました.
結論:
- * E. crassus Tec1 要素は,マクロ核発達の過程でゲノムから精密な切除を受けます.
- * 切除はプロセス初期に発生し,DNA複製とポリテニウム形成と一致します.
- * このプログラムされたDNAの除去は,体内マクロ核ゲノムにTec1が存在しないことを保証します.
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