遠く離れた種のトランスクリプトームの比較分析
Mark B Gerstein1, Joel Rozowsky2, Koon-Kiu Yan2
11] Program in Computational Biology and Bioinformatics, Yale University, Bass 432, 266 Whitney Avenue, New Haven, Connecticut 06520, USA [2] Department of Molecular Biophysics and Biochemistry, Yale University, Bass 432, 266 Whitney Avenue, New Haven, Connecticut 06520, USA [3] Department of Computer Science, Yale University, 51 Prospect Street, New Haven, Connecticut 06511, USA [4] [5].
Nature
|August 29, 2014
まとめ
種間のトランスクリプトームを比較すると,基本的な生物学的原理が明らかになる. この研究は,共有された遺伝子発現パターンと,ヒト,虫,ハエの染色体データから遺伝子活動を予測するための普遍的なモデルを特定しました.
科学分野:
- 比較ゲノミクスとは
- トランスクリプトミクス (Transcriptomics) とは
- 発達生物学 発達生物学とは
背景:
- トランスクリプトームは,ゲノム活動のスナップショットを提供します.
- 種間トランスクリプトームの比較は,基本的な生物学的原理を明らかにすることができます.
- 以前の比較は,種または属の範囲に限られていた.
研究 の 目的:
- 遠い動物種 (人間,虫,ハエ) のトランスクリプトームに保存された特徴を識別する.
- 染色体データから遺伝子発現を予測するための普遍的なモデルを開発する.
- 種間の非コーディングトランスクリプションレベルを比較する.
主な方法:
- ENCODEとmodENCODEコンソーシアムによるRNAシーケンシングデータ生成と統一処理.
- 人間,虫,ハエのトランスクリプトームの比較分析.
- クロマチン特性を用いた予測モデルの開発と応用.
主要な成果:
- 共同発現モジュールの発見,多くの開発遺伝子が豊かにされ,メタゾーンフィラ全体で共有されています.
- ワーム・プーパとハエの胚の間の新しい発達段階の調整の特定.
- 種間の塩基対あたりの類似した非正規の転写レベルの定量化.
- 遺伝子発現レベルがプロモータークロマチンの特徴から生物独立モデルを用いて予測できることを実証.
結論:
- 異なった種のトランスクリプトームを比較すると,古くから保存されている特徴が明らかになる.
- 発達過程は,動物全体で保存された規制原理を示しています.
- 普遍的なモデルは,種間のクロマチンのデータから遺伝子発現を予測し,保存された規制メカニズムを強調することができます.
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