発芽中心のB細胞の選択中にT卵泡ヘルパー細胞によるダイナミックシグナル伝達

Ziv Shulman¹, Alexander D Gitlin¹, Jason S Weinstein²

¹Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.

Science (New York, N.Y.)

|August 30, 2014

まとめ

T毛細葉ヘルパー (T(FH)) 細胞は,大きく,一時的な接触を通して,生殖中心のB細胞と相互作用します. これらのカルシウム媒介の相互作用は,抗体生成のためのB細胞の選択を駆動する.

科学分野:

免疫学免疫学とは
細胞生物学細胞生物学
分子生物学は分子生物学である.

背景:

T卵泡ヘルパー (T(FH)) 細胞は,生殖中心 (GC) でのB細胞の選択に不可欠です.
T ((FH) とGC B細胞の相互作用を制御する正確なメカニズムは,まだ完全に理解されていません.
これらの相互作用を理解することは,抗体生産と免疫記憶を解読する鍵です.

研究の目的:

B細胞の選択中のT (((FH) 細胞とGC B細胞のダイナミックな相互作用を解明する.
細胞間カルシウムシグナル伝達の作用を研究するために,T (((FH) 細胞媒介選択.
細胞と細胞の接触の性質と期間を特徴付けるために.

主な方法:

生きた動物における生殖中心の腸内イメージング.
T(FH) 細胞の細胞内自由カルシウム (Ca2+) ダイナミクスの分析.
T (((FH) 細胞におけるサイトカイン共発現 (インタールイキン-4および-21) の評価.
トラッキングT(FH) 細胞移動パターンと接触期間.

主要な成果:

選択は,高レベルの同類ペプチド-MHC II.を呈するT(FH) とGC B細胞の間の大きく,一時的な接触によって媒介されます.
これらの相互作用は,細胞内Ca2+のT(FH) の一時的および持続的な増加を誘導する.
増加したCa2+はT(FH) 細胞の移動を止めなかった. 細胞は動き続け,複数のGC B細胞をスキャンした.
高 Ca2+ の短期的な接触が繰り返し,B細胞の選択を促す.

結論:

高親和性B細胞のT ((FH) 細胞選択は,安定した結合体よりも,動的,カルシウム依存の相互作用を伴う.
移動性T (FH) 細胞は,GC B細胞を継続的に調査し,選択のために繰り返される一時的なカルシウム信号を使用します.
このダイナミックなスキャンメカニズムは,最適な抗体生成B細胞の効率的な選択を保証します.

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