Sxl-Unr翻訳規制複合体の構築のための構造的基礎
Janosch Hennig1, Cristina Militti2, Grzegorz M Popowicz1
11] Institute of Structural Biology, Helmholtz Zentrum München, Ingolstädter Landstrasse 1, DE-85764, Germany [2] Center for Integrated Protein Science Munich at Biomolecular NMR Spectroscopy, Department Chemie, Technische Universität München, Lichtenbergstr. 4, DE-85747 Garching, Germany.
Nature
|September 12, 2014
まとめ
Sex-lethal (Sxl) および Upstream-of-N-ras (Unr) タンパク質は,msl2 mRNAに結合し,雌果物ハエの投与補償を抑制する. この分子スイッチは,ユニークな相互作用を通じて翻訳を調節することによって,女性の生存能力を確保します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 構造生物学 構造生物学とは
背景:
- 投与量の補償は,X関連遺伝子発現を男女間で均等にする.
- ドロソフィラ・メラノガスターでは,これは男性X染色体のハイパートランスクリプションを伴う.
- 女性特異性Sex-lethal (Sxl) タンパク質は,msl2翻訳を阻害することによって,用量補償を沈黙させます.
研究 の 目的:
- Sxl と Unr.による msl2 翻訳抑制の構造的基礎を解明する.
- メス果物ハエの用量補償サイレンシングの分子機構を理解するために.
主な方法:
- X線結晶学 (2.8 Å 解像度)
- 核磁共振 (NMR) スペクトロスコーピーは,核磁共振 (NMR) スペクトロスコーピーのスペクトロスコーピーを用います.
- スモールアングルX線と中性子散射 (SAXS/SANS)
主要な成果:
- 立体Sxl-Unr-msl2リボ核タンパク質複合体の構造を決定しました.
- Sxl RNAの認識モチーフ,Unrの冷凍ショック領域,およびRNAの間の前例のない交絡した相互作用が明らかになりました.
- 協力的な複合体の形成が実証され,Unr RNAの結合親和性が1000倍に増加しました.
- 新しい三元相互作用と非正規のRNA接触を特定しました.
結論:
- Sxl-Unr-msl2複合体は,リボ核タンパク質のスイッチとして働き,翻訳を調節する.
- 特定の分子相互作用が,女性生存に不可欠な,用量補償抑制を誘発する.
- このメカニズムは,多様なRNA結合ドメインが,遺伝子調節のための配列特異的なRNA認識を拡大する方法を強調しています.
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