高親和性IgE受容体のトランスフェクト細胞における完全な構造と発現
1Section on Chemical Immunology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, Bethesda, Maryland 20892.
Nature
|January 12, 1989
まとめ
研究者らは,アレルギー反応に不可欠な高親和性免疫グロブリンE受容体 (Fc epsilon RI) のガンマサブユニットをクローンした. すべてのサブユニットの共同発現により,受容体の機能が可能になり,ヒトのFcエプシロンRI相互作用の研究への道が開けました.
科学分野:
- 免疫学 免疫学とは
- 分子生物学は分子生物学である.
- アレルギー研究 アレルギー研究
背景:
- 高親和性免疫グロブリンE受容体 (Fc epsilon RI) は,アレルギー反応の鍵であり,マスト細胞とベースフィールからメディエーターの放出を媒介する.
- FcエプシロンRIはネズミのテトラメリック複合体 (アルファ,ベータ,ガンマ2) で,IgE結合と信号伝導に不可欠です.
- 以前の研究では,αおよびβサブユニットcDNAを分離したが,IgE結合発現を達成できなかった.
研究 の 目的:
- Fc epsilon RI.のガンマサブユニットをコードする補完DNA (cDNA) をクローンする.
- FcエプシロンRIテトラマー (アルファベータガンマ2) の構造モデルを提案する.
- 感染した細胞でFcエプシロンRIの機能的発現を達成するために.
主な方法:
- FcエプシロンRIガンマサブユニットの補完DNA (cDNA) クローン.
- COS 7細胞とアルファ,ベータ,ガンマサブユニットをコードするcDNAの共感染.
- FcエプシロンRIアルファベータガンマ2テトラメールの構造モデリング.
主要な成果:
- Fc epsilon RI gammaサブユニットcDNAのクローニングが成功しました.
- COS 7細胞における歯類Fc epsilon RIの発現は,3つのサブユニットcDNAの共伝染によってのみ達成された.
- アルファベータガンマ2テトラメアの構造モデルが提案され,受容体特性を説明しました.
結論:
- ガンマ亜単位は,FcエプシロンRIの機能表現に不可欠である.
- すべてのFcエプシロンRIサブユニットの共同発現は,受容体の組立と機能のために必要である.
- この研究は,ヒトFcエプシロンRIの研究と,アレルギー性疾患に対する標的型阻害剤の開発を可能にします.
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