Jove
Visualize
お問い合わせ
JoVE
x logofacebook logolinkedin logoyoutube logo
JoVEについて
概要リーダーシップブログJoVEヘルプセンター
著者向け
出版プロセス編集委員会範囲と方針査読よくある質問投稿
図書館員向け
推薦の声購読アクセスリソース図書館諮問委員会よくある質問
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experimentsアーカイブ
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教員リソースセンター教員サイト
利用規約
プライバシーポリシー
ポリシー

関連する概念動画

Exon Recombination02:32

Exon Recombination

3.1K
The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
Exon shuffling follows “splice frame rules.” Each exon...
3.1K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.7K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.7K
RNA Splicing01:32

RNA Splicing

53.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
53.2K
Gene Conversion02:08

Gene Conversion

9.2K
Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
9.2K
Proofreading01:31

Proofreading

7.5K
Synthesis of new DNA molecules is carried out by the enzyme DNA polymerase, which adds nucleotides on the daughter strand complementary to the template DNA strand. DNA polymerase has a higher affinity to add the correct base and ensures fidelity during DNA replication. Furthermore,  it exhibits proofreading activity during replication, using an exonuclease domain that cuts off incorrect nucleotides from the nascent DNA strand.
Errors During Replication are Corrected by the DNA Polymerase...
7.5K
Homologous Recombination02:31

Homologous Recombination

58.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.6K

こちらも読む

関連記事

共著者、ジャーナル、引用グラフによってこの研究に関連する記事。

並び替え
Same author

Clinical Classification of Buffalo Hump Based on Appearance and MRI: A Retrospective Study of 383 Cases.

Aesthetic plastic surgery·2026
Same author

Multidisciplinary management promoting wound healing in a patient with Proteus syndrome: a case report.

Frontiers in pediatrics·2026
Same author

Defining the Ideal Cranial Vault Height Ratio Via Morphometric Analysis: A Preliminary Study.

Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]·2026
Same author

Retrospective Study on Palpable Indurations After Cosmetic Filler Injection: Ultrasonic Characterization and Image-Guided Therapy.

Aesthetic surgery journal·2026
Same author

Efficacy and acupoint specificity of sedative-tranquilizing acupuncture for Alzheimer's disease with sleep disorders: A three-arm randomized controlled trial protocol.

Complementary therapies in medicine·2026
Same author

Coordination-driven supramolecular metalla-cycles/cages for next-generation antibacterial therapy.

Chemical communications (Cambridge, England)·2026

関連する実験動画

Updated: Apr 23, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

6.8K

補完的な配列媒介によるエクソン循環化.

Xiao-Ou Zhang1, Hai-Bin Wang2, Yang Zhang3

  • 1Key Laboratory of Computational Biology, CAS-MPG Partner Institute for Computational Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China.

Cell
|September 23, 2014
PubMed
まとめ

哺乳類におけるエクソン循環化は,隣接するイントロンの補完的な配列に依存しています. このプロセスは進化的にダイナミックで,単一の遺伝子から複数の円形RNAトランスクリプトを生成することができます.

さらに関連する動画

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

28.3K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.7K

関連する実験動画

Last Updated: Apr 23, 2026

Use of Alu Element Containing Minigenes to Analyze Circular RNAs
13:10

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

6.8K
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
14:26

Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells

Published on: April 4, 2016

28.3K
CIRCLE-Seq for Interrogation of Off-Target Gene Editing
08:23

CIRCLE-Seq for Interrogation of Off-Target Gene Editing

Published on: November 1, 2024

1.7K

科学分野:

  • 分子生物学は分子生物学である.
  • ゲノミクスゲノミクスとは
  • RNA 生物学 RNA 生物学

背景:

  • エクソン循環化は哺乳類で観察されているが,その生体生成のメカニズムは不明である.
  • 円形のRNA形成を理解することは,転写後の調節に関する洞察にとって極めて重要です.

研究 の 目的:

  • エクソン循環化バイオゲネシスのメカニズムを解明する.
  • エクソン循環化の進化的ダイナミクスと規制メカニズムを調査する.

主な方法:

  • エクソン循環化を研究するために全ゲノムアプローチが採用されました.
  • 循環型RNA復習実験が行われました.

主要な成果:

  • エクソン循環化は,隣接するイントロニック補完配列に依存している.
  • これらの配列は急速に進化し,ダイナミックなエクソン循環化を示している.
  • RNA ペアリングの間の競争は,循環化の効率に影響します.
  • 代替の反転繰り返しのAluペアは,代替の循環化につながり,一つの遺伝子から複数の円形RNAを生成します.

結論:

  • 人間のイントロンの補完的な配列は,エクソン循環化を媒介する.
  • このメカニズムは,哺乳類の転写後の調節に複雑さを加えている.
  • 代替的循環化は,RNAトランスクリプトのレパートリーを拡大する.