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関連する概念動画

CRISPR01:59

CRISPR

46.2K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
46.2K
CRISPR01:59

CRISPR

13.7K
13.7K
CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

3.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
3.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

14.5K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
14.5K
The Antiviral System of Bacteria and Archaea: CRISPR01:23

The Antiviral System of Bacteria and Archaea: CRISPR

1.0K
CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
1.0K
Homologous Recombination02:31

Homologous Recombination

58.6K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
58.6K

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関連する実験動画

Updated: Apr 23, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
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プログラム可能なRNAの認識とCRISPR/Cas9による分裂.

Mitchell R O'Connell1, Benjamin L Oakes1, Samuel H Sternberg2

  • 1Department of Molecular and Cell Biology, University of California, Berkeley, California 94720, USA.

Nature
|October 3, 2014
PubMed
まとめ

CRISPR-Cas9は,DNAだけでなく,RNAをターゲットにすることができます. 独立したDNA配列 (PAMmer) を使用することで,Cas9は特定のRNA分子に結合して切断することができ,プログラム可能なトランスクリプト認識を可能にします.

さらに関連する動画

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System

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関連する実験動画

Last Updated: Apr 23, 2026

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells
11:35

Selection-dependent and Independent Generation of CRISPR/Cas9-mediated Gene Knockouts in Mammalian Cells

Published on: June 16, 2017

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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Efficient Generation of hiPSC Neural Lineage Specific Knockin Reporters Using the CRISPR/Cas9 and Cas9 Double Nickase System
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科学分野:

  • 分子生物学は分子生物学である.
  • 遺伝学 遺伝学とは
  • バイオテクノロジー バイオテクノロジー

背景:

  • CRISPR関連タンパク質9 (Cas9) は,RNA互補性およびプロト空間器の隣接モチーフ (PAM) による標的認識を導くDNA内核酵素である.
  • Cas9はゲノム工学で広く使用されていますが,RNAをターゲットにすることができないと考えられました.
  • Cas9の基板特異性を理解することは,そのアプリケーションの拡大に不可欠です.

研究 の 目的:

  • Cas9がPAM配列の助けを借りて単一鎖RNA (ssRNA) を標的にできるかどうかを調査する.
  • Cas9-RNAの相互作用と分裂のメカニズムを探求する.
  • 生物学的文脈でRNAターゲティングのためのCas9の有用性を実証する.

主な方法:

  • Cas9,ガイドRNA,ssRNA標的,およびトランスプレゼンテッドPAMオリゴヌクレオチド (PAMmers) を使用したインビトロアッセイ.
  • PAMマーによるssRNA標的に対するCas9結合親和性の特徴.
  • PAMmersによって刺激されたサイト固有のssRNA分裂の実証.
  • 細胞から内生メッセンジャーRNA (mRNA) を分離する方法の適用.

主要な成果:

  • Cas9は,PAM配列がDNAオリゴヌクレオチド (PAMmer) 経由でトランスで提供されると,ssRNA標的に高い親和性で結合する.
  • PAMmersは,sssRNA標的のCas9媒介,サイト固有のエンドヌクレオリチス分裂を刺激する.
  • Cas9は,特に設計されたPAMmersを使用してDNA配列を回避しながらRNAターゲットを指向することができます.
  • この戦略により,細胞から特定の内在的なmRNAを隔離することが成功しました.

結論:

  • Cas9のPAM結合は,基本的にその基板選択と関連しており,RNAターゲティングを可能にします.
  • Cas9は,ターゲットタグを必要とせずに,特定のトランスクリプト認識と分割のためにプログラムすることができます.
  • この研究は,Cas9の有用性をDNAを超えて拡大し,RNAの操作と分析のための新しい道を開きます.