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関連する概念動画

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

8.0K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
8.0K
Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K
Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

2.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
2.4K
Fusion of Secretory Vesicles with the Plasma Membrane01:26

Fusion of Secretory Vesicles with the Plasma Membrane

15.8K
Proteins and neurotransmitters in secretory vesicles can be released from a cell upon vesicle docking, priming, and fusion with the plasma membrane. Vesicles are docked and primed in preparation for the quick exocytosis of their contents in response to a stimulus. The fusion process is mainly carried out by a SNAP Receptor or SNARE complex, consisting of synaptobrevin, syntaxin-1, and SNAP-25.
In 1993, Jim Rothman proposed that the antiparallel pairing of vesicular and transmembrane SNAREs, or...
15.8K
Delivery Pathways to the Lysosome01:36

Delivery Pathways to the Lysosome

7.6K
Eukaryotic cells use different mechanisms to eliminate toxic waste obsolete and worn-out substances. Lysosomes play a pivotal role in this, and hence, these substances are carried to the lysosome from other parts of the cell and extracellular space through different pathways. The most elaborately studied pathways to the lysosome are the endocytic pathways.
Endocytosis
In endocytosis, the cell membrane takes up macromolecules and particles from the surrounding medium. Clathrin-mediated...
7.6K
Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

2.7K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
2.7K

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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
07:30

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

Published on: September 4, 2017

9.6K

クラトリンは,エンドソームからシナプスベジクルを再生します.

Shigeki Watanabe1, Thorsten Trimbuch2, Marcial Camacho-Pérez2

  • 1Department of Biology and Howard Hughes Medical Institute, University of Utah, Salt Lake City, Utah 84112-0840, USA.

Nature
|October 9, 2014
PubMed
まとめ
この要約は機械生成です。

超高速エンドサイトーシスは,シナプスを迅速に回収する. クラトリンは,エンドソームからのシナプス везикулの再生に不可欠であり,最初の超高速回収には欠かせません.

さらに関連する動画

Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish
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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

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An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
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関連する実験動画

Last Updated: Apr 22, 2026

Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons
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Measuring Synaptic Vesicle Endocytosis in Cultured Hippocampal Neurons

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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish
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Live Imaging of Synaptic Vesicle Recycling in the Neuromuscular Junction of Dissected Larval Zebrafish

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An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins
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An Optical Assay for Synaptic Vesicle Recycling in Cultured Neurons Overexpressing Presynaptic Proteins

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科学分野:

  • 神経科学は神経科学である.
  • 細胞生物学 細胞生物学
  • 分子生物学は分子生物学である.

背景:

  • シナプス水泡のリサイクルは,神経細胞のコミュニケーションに不可欠です.
  • シナプス膀エンドサイトーシスのメカニズムとタイミングは,まだ議論されている.
  • 超高速エンドサイトーシスは,溶融後の大きな膀を迅速に回収する.

研究 の 目的:

  • 超高速エンドサイトーシスで形成される大きな内細胞胞の運命を明らかにする.
  • シナプス膀のリサイクルにおけるクラトリンとアクチンの役割を決定する.
  • エンドサイトーシスにおけるクラトリンの役割に関する公表された研究における不一致を解決する.

主な方法:

  • RNA干渉 (RNAi) を利用してクラスリン機能を妨害した.
  • 操作されたアクチンポリメリゼーションと神経刺激温度.
  • 時間とともに,内細胞小胞のシナプス小胞への移行を追跡した.

主要な成果:

  • 大型の内細胞胞は1秒以内にシナプスエンドソームに成熟します.
  • エンドソームは5〜6秒以内に,コーティングされた膀,そして小さなシナプス膀に溶解します.
  • クラトリンは,エンドソームからシナプスベジクルを生成するために必要ですが,超高速エンドサイトーシスには必要ありません.
  • 超高速エンドサイトーシスは,アクチンポリメリゼーションと生理学的温度に依存します.
  • クラトリン媒介性内分細胞症は,超高速内分細胞症が損なわれるときに,代替的な回収経路として機能します.

結論:

  • 超高速エンドサイトーシスは,最初の膀回収のためのクラトリン独立経路です.
  • クラトリン依存性内分細胞化は,エンドソームからシナプス胞の後の再構成に不可欠である.
  • アクチンのポリメリゼーションと温度は,超高速エンドサイトーシスの重要な要因です.
  • この発見は,異なるシナプス水泡内分細胞分裂経路におけるクラトリンの異なる役割を明らかにしている.