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Updated: Apr 22, 2026

CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery
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CRISPR Epigenome Editing in Human Cells using Plasmid DNA Transfection and mRNA Nucleofection Delivery

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ゲノムスケールのCRISPR媒介による遺伝子抑制と活性化の制御

Luke A Gilbert1, Max A Horlbeck1, Britt Adamson1

  • 1Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA; Howard Hughes Medical Institute, University of California, San Francisco, San Francisco, CA 94158, USA; California Institute for Quantitative Biomedical Research, San Francisco, CA 94158, USA; Center for RNA Systems Biology, University of California, San Francisco, San Francisco, CA 94158, USA.

Cell
|October 14, 2014
PubMed
まとめ

CRISPR干渉 (CRISPRi) とCRISPR活性化 (CRISPRa) ツールを開発し,トランスクリプトレベルを正確に制御することによって遺伝子機能を体系的に研究しました. これらの強力な方法は,健康と病気における細胞経路と遺伝子の役割を明らかにします.

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