テラトカルシノーマ細胞におけるIgH増強剤の抑制は,新しいオクタマー因子と関連している

M J Lenardo¹, L Staudt, P Robbins

¹Whitehead Institute for Biomedical Research, Cambridge, MA 02142.

Science (New York, N.Y.)

|January 27, 1989

まとめ

胚性癌 (EC) 細胞は,他の細胞とは異なり,特定のDNA配列を抑制するために,ユニークなタンパク質であるNF-A3を使用します. このタンパク質は,

科学分野:

発達生物学発達生物学とは
分子生物学は分子生物学である.
遺伝子調節遺伝子調節

背景:

胚性癌 (EC) 細胞系は,マウスの早期胚形成のモデルとして機能しています.
特定の重鎖増強剤の断片は,他の細胞タイプと比較して,F9 EC細胞の活性が低下していることを示しています.

研究の目的:

EC細胞における増強剤の活性を管理する規制メカニズムを調査する.
F9 EC細胞における重鎖増強剤の異なる活性に起因する要因を特定する.

主な方法:

F9 EC細胞における300塩基対重鎖強化剤の断片の分析.
オクタマーモチーフの変化に伴う強化剤の活性度の評価.
F9細胞におけるオクトマー結合タンパク質を特定するための核抽出分析.

主要な成果:

重鎖増強剤の断片は,F9 EC細胞では無活性であったが,他の非リンパ球細胞では活性であった.
オクタマーモチーフの改変により,F9細胞における増強剤の活性が増加した.
EC細胞に特有の新しいオクトーマー結合タンパク質,NF-A3が特定され,分化時に減少することが判明しました.

結論:

EC細胞特異のタンパク質であるNF-A3は,オクターマーモチーフを含む規制配列を抑制しているようです.
同じDNA配列は,細胞のタイプによって,陰性と陽性の両方の転写効果を媒介することができます.

さらに関連する動画

08:35

Inducible and Reversible Dominant-negative (DN) Protein Inhibition

Published on: January 7, 2019

10:10

HOX Loci Focused CRISPR/sgRNA Library Screening Identifying Critical CTCF Boundaries

Published on: March 31, 2019

関連する概念動画

02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...

02:36

RNA Polymerase II Accessory Proteins

Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...

01:52

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...

02:33

Hedgehog Signaling Pathway

The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...

01:30

General Transcription Factors

01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012 for this...

関連する実験動画