膀輸送の単一ラウンドの解剖:ゴルギのスタックにおけるインターシステナル移動のための連続的な中間物質
L Orci1, V Malhotra, M Amherdt
1Department of Morphology, University of Geneva Medical School, Switzerland.
Cell
|February 10, 1989
まとめ
この研究は,細胞のないシステムを用いて,ゴルジのキャリアベジクルの成熟の異なる段階を明らかにしています. コーティングされたおよびコーティングされていない膀は,タンパク質の密輸における連続的な輸送中介物として識別されます.
科学分野:
- 細胞生物学 細胞生物学
- 分子生物学は分子生物学である.
- タンパク質の輸送
背景:
- ゴルギ装置は,タンパク質の分泌または他の臓器への配送を修正し,分類するために不可欠です.
- ゴルギの内部での膀輸送のダイナミクスを理解することは,細胞タンパク質の密輸経路の解読に不可欠です.
研究 の 目的:
- ゴルギ内輸送中のキャリアベシクルの成熟の離散的段階を特定し,特徴づけること.
- ゴルギ水槽間のVSV-Gタンパク質の輸送におけるコーティングされたおよびコーティングされていない膀の役割を解明する.
主な方法:
- セルフリー・システムを活用し,ゴルギの水槽間でのVSV-Gタンパク質の1回の輸送を復元した.
- 選択的GTPγS阻害剤とN-エチルマレイミド (NEM) を使用して,それぞれコーティングされたおよびコーティングされていない膀を蓄積します.
- 適切に治療された全細胞で確認された結果.
主要な成果:
- 連続的かつ必須の輸送中介物質として,コーティングされたおよびコーティングされていないベシクルを識別した.
- 蓄積された膀が融合を阻害されているが,ゴルギのドナーと受容体スタックの間に移転していたことを実証した.
- 細胞フリー系と全細胞系の両方で,類似した膀群を観察した.
結論:
- コーティングされた芽コーティングされたベジクル-コーティングされていないベジクルシステムを提案しました. ゴルギのスタック全体に生物合成タンパク質輸送のためのベジクルシステムは,内分細胞症に類似しています.
- "ゴルギ"で覆われた芽にはクラトリンがなく,選択性エンドサイトクラトリンで覆われたピットとは異なり,バルクキャリアとして機能することを強調しました.
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