細胞粘着分子ICAM-1は,ライノウイルスの主要な表面受容体です
D E Staunton1, V J Merluzzi, R Rothlein
1Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|March 10, 1989
まとめ
風邪を誘発する主要群のライノウイルスは,受容体として細胞間粘着分子1 (ICAM-1) を利用する. この受容体は,リンパ球機能関連分子1 (LFA-1) によっても使用され,共通の結合部位を示唆しています.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 免疫学 免疫学とは
- 細胞生物学 細胞生物学
背景:
- リノウイルスは風邪を誘発し,100種類以上の血清型を持っています.
- リンパ球機能関連分子1 (LFA-1) は,細胞間粘着分子1 (ICAM-1) と結合することによって白血球の粘着を媒介する.
研究 の 目的:
- 主要なライノウイルスのグループで使用される受容体を特定するために.
- リノウイルス受容体とICAM-1との関係を調査する.
主な方法:
- 浄化されたICAM-1とICAM-1をトランスフェクトされた細胞で表した結合測定法.
- ICAM-1 特定のモノクローナル抗体 (MAbs) を用いた抑制研究.
- HeLa細胞におけるライノウイルス誘発のサイトパシー効果の評価.
主要な成果:
- メジャーグループライノウイルスの受容体はICAM-1として特定されました.
- ライノウイルスによるICAM-1への結合は,LFA-1結合を阻害するMAbsによって阻害され,近隣または同一の結合部位を示した.
- ICAM-1 MAbsは,主要な,しかしマイナーではない,グループライノウイルスの効果をブロックしました.
結論:
- ICAM-1はメジャーグループライノウイルスの受容体として機能する.
- LFA-1とリノウイルスに対するICAM-1の共通の結合部位は,ウイルスの侵入と免疫細胞の相互作用の共通のメカニズムを示唆しています.
- 炎症誘発によるICAM-1の発現は,ライノウイルス感染の拡大を促進する可能性があります.
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