異なるT3結合能力を持つタンパク質をコードする2つのerbAホモログは,同じ遺伝的場所の対極のDNA鎖から転写される
N Miyajima1, R Horiuchi, Y Shibuya
1Department of Oncology, University of Tokyo, Japan.
Cell
|April 7, 1989
まとめ
人間の2つの遺伝子,ear-1とear-7は,甲状腺ホルモン受容体に関連するタンパク質をコードする. Ear-7は2つの同型を生成し,ear71は甲状腺ホルモン (T3) に強く結合し,ear-1はより弱い結合を示している.
科学分野:
- 遺伝学 遺伝学とは
- 分子生物学は分子生物学である.
- エンドクリノロジー エンドクリノロジー
背景:
- 人間のゲノムには,ErbAの2つの同種であるEAR-1とEAR-7が,染色体17に位置しています.
- これらの遺伝子は遺伝的位置を共有し,重複するエクソンを特徴とし,対極のDNA鎖から転写されます.
研究 の 目的:
- ear-1およびear-7遺伝子産物の特性および機能を調査する.
- 特定されたタンパク質イソフォームの甲状腺ホルモン (T3) 結合能力を決定する.
主な方法:
- タンパク質の構造を予測するための核酸配列分析.
- インビトロタンパク質合成.
- T3結合親和性を評価するためのScatchard分析.
主要な成果:
- ear-7遺伝子は代替スプライシングを行い,ear71とear72のタンパク質同型を形成する.
- ear71タンパク質は,甲状腺ホルモン受容体と一致するT3への高親和結合を示す.
- ear72タンパク質はT3.3を結合しない.
- ear-1遺伝子の産物は,低,しかし検出可能なT3結合を示しています.
結論:
- ear71タンパク質は,甲状腺ホルモン受容体として機能する,c-erbAタンパク質のヒトの対照として識別されています.
- ear-1遺伝子産物もT3と相互作用するが,その主要リガンドについてはさらなる解明が必要である.
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