分子ルールは,真核細胞のシリアとフラゲラの繰り返しの長さを決定します

Toshiyuki Oda¹, Haruaki Yanagisawa¹, Ritsu Kamiya²

¹Department of Cell Biology and Anatomy, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo Bunkyo-ku, Tokyo, 113-0033, Japan.

Science (New York, N.Y.)

|November 15, 2014

まとめ

科学者たちは,真核細胞のとフラゲラに分子ルールを発見した. FAP59/172複合体は,これらの重要な細胞構造における96ナノメートルの重複長と構成要素の配置を決定する.

科学分野:

細胞生物学細胞生物学
分子生物学は分子生物学である.
バイオフィジックス生物物理学

背景:

細胞は細胞構造の大きさを調節するメカニズムを持っているが,真核生物における長さの決定の分子基盤はほとんど不明である.
分子支配者は細胞の次元を制御すると仮定されていますが,真核系における具体的な例は稀です.

研究の目的:

ユカリオットのシリアとフラジェラにおける長さの決定の基礎となる分子機構を特定し,特徴づけること.
特定のタンパク質複合体の作用を調査し,アクソネーム内の重複する構造単位を確立する.

主な方法:

クライオエレクトロントモグラフィーは,クロミドモナスのフラゲラ内のタンパク質複合体の高解像度構造を視覚化するために使用されました.
生物化学的および遺伝的アプローチを使用して,特定されたタンパク質複合体の機能がアクソネームの組立と長さの調節における機能を評価しました.

主要な成果:

FAP59とFAP172のタンパク質は,クロミドモナスのフラゲル内で96ナノメートル (nm) の長さの複合体を形成します.
FAP59/172複合体の削除は,軸膜構造の特徴的な96nmの繰り返しを破壊しました.
延長されたFAP59/172複合体のエンジニアリングは,延長された繰り返しの長さ (128 nmまで) と変異した軸索膜構成要素の組織につながりました.

結論:

FAP59/172複合体は,シリアとフラゲラにおける96nmの繰り返し長さを定義する分子ルラーとして機能します.
この複合体は,アクソネーム内のコンポーネントの正確な配置に不可欠であり,適切な構造と機能を保証します.

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