推定のマウリン・エコトロピック・レトロウイルス受容体遺伝子は,複数の膜を網羅するタンパク質をコードし,ウイルス感染に対する感受性を授与する
L M Albritton1, L Tseng, D Scadden
1Howard Hughes Medical Institute, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|May 19, 1989
まとめ
研究者らは,エコトロピックレトロウイルスの細胞への侵入に不可欠な新しいマウス受容体を特定しました. この発見により,レトロウイルス感染症のメカニズムと潜在的な治療標的の理解が進んでいます.
科学分野:
- ウイルス学 ウイルス学 ウイルス学
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
背景:
- マウスのC型エコトロピックレトロウイルス感染症は,マウスの細胞の特定の膜受容体と結合するウイルス封筒に依存しています.
- この受容体を特定することは,レトロウイルスの侵入メカニズムを理解するために非常に重要です.
研究 の 目的:
- エコトロピックレトロウイルス結合と感染に責任を負うマウスの膜受容体を特定し,特徴づけること.
主な方法:
- マウスNIH 3T3DNAの遺伝子移植をヒトEJ細胞に活用しました.
- 薬剤耐性遺伝子を運ぶレトロウイルスベクターに耐性のあるEJ細胞クローンに選択されています.
- ネズミの繰り返しDNA配列との結合により,推定受容体cDNAクローンを特定した.
主要な成果:
- 特定されたcDNAを発現するヒトEJ細胞は,ネズミ白血病ウイルス (MuLV) の感染性の100万倍の増加を示しました.
- 予測された622アミノ酸受容体タンパク質は,14の潜在的な膜を網羅するドメインを持つ高度な水害性である.
- 配列分析は,既知のタンパク質と有意な類似性を明らかにせず,新しい受容体を示唆しました.
結論:
- 新しい膜タンパク質が,エコトロピー型 MuLV感染に対する感受性を決定する受容体として作用する.
- この受容体は,ウイルスの封筒との結合および/または融合を通じてウイルスの侵入を媒介する可能性があります.
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