関連する実験動画

Updated: Jul 9, 2026

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Transient Expression of Proteins by Hydrodynamic Gene Delivery in Mice

Published on: May 5, 2014

Mov13マウスのレトロウイルス誘発の挿入変異は,組織特異的な方法でコラーゲンI発現に影響します

K Kratochwil¹, K von der Mark, E J Kollar

¹Institute of Molecular Biology, Austrian Academy of Sciences, Salzburg.

|June 2, 1989

まとめ

Mov13マウス変異体では,コラーゲン遺伝子転写がブロックされています. しかし,これらの変異体の歯は,オドントブラストの機能的なmRNA生成による正常なコラーゲン1レベルを示しています.

科学分野:

分子生物学は分子生物学である.
遺伝学遺伝学とは
発達生物学発達生物学とは

背景:

Mov13マウス突然変異は,レトロウイルス挿入により,α1 (1) コラーゲン遺伝子の転写がブロックされていることを示しています.
コラーゲンI型は,歯類を含む様々な組織の構造的整合性にとって極めて重要です.

研究の目的:

オドントブロストにおけるコラーゲン遺伝子発現に対するレトロウイルスインサートの効果を調査する.
Mov13変異が歯のコラーゲン産生に影響を与えるメカニズムを決定する.

主な方法:

特定の細胞タイプにおける遺伝子転写を検出するために,in situハイブリデーションを行う.
mRNAレベルを定量化するためにRNAase保護アッセイ.
ミュータント胚のデンチンのコラーゲン1含有量の分析.

主要な成果:

ホモジゴス Mov13の胚は,正常量のコラーゲンを含む歯茎を産生した 1.
変異したアルファ1 (1) コラーゲンアルレルは,線維芽細胞と異なり,オドントブラストに効率的に転写されました.
機能的なα1 (1) コラーゲンmRNAは,ウイルス配列の正しいスプライシングによって生成されました.

結論:

Mov13変異体のレトロウイルス挿入は,オドントブラストにおけるコラーゲン1の産生を阻害しません.
挿入は,一般的な転写ではなく,組織特異の転写制御に干渉する可能性があります.
これは,オドントブラストの細胞型特異的なシス作用の調節要素の不活性化を示唆している.

さらに関連する動画

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Enhanced Crosslinking Immunoprecipitation (eCLIP) Method for Efficient Identification of Protein-bound RNA in Mouse Testis

Published on: May 10, 2019

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

関連する概念動画

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

Point and Frameshift Mutations

Point and Frameshift Mutations

Point mutations are genetic alterations involving the change of a single nucleotide base pair in DNA. Depending on how the alteration affects protein synthesis, they can lead to various consequences.Point mutations fall into the following types:Silent mutations occur when a nucleotide change does not alter the amino acid sequence due to the redundancy of the genetic code. For instance, changing ACC to ACA still encodes threonine, leaving the protein function unaffected. This occurs because...