エプスタイン・バーウイルス小RNA (EBER) 遺伝子:RNAポリメラーゼIIとIIIのプロモーター要素を組み合わせたユニークな転写単位
1Howard Hughes Medical Institute, Department of Molecular Biophysics and Biochemistry, New Haven, Connecticut 06510.
Cell
|June 2, 1989
まとめ
エプスタイン・バーウイルスにコードされた小RNA (EBER) 遺伝子は,ATFとSp1結合部位を含むユニークなプロモーター要素を高レベルの発現のために利用します. これらの発見は,EBVが変異した細胞におけるRNAポリメラーゼIII転写の新たな規制メカニズムを明らかにしています.
科学分野:
- 分子生物学は分子生物学である.
- ウイルス学 ウイルス学 ウイルス学
- 遺伝子規制 遺伝子規制
背景:
- エプスタイン・バーウイルスにコード化された小型のRNA (EBER) は,感染した細胞に豊富に存在するトランスクリプトです.
- 彼らの正確な転写調節とプロモーター要素は,まだ完全に理解されていません.
- EBERは,RNAポリメラーゼIIIトランスクリプトのユニークなクラスを表しています.
研究 の 目的:
- EBER遺伝子発現を制御するプロモーター要素と転写因子の解明.
- EBV変換リンパ球におけるEBERの高レベルの発現の背後にあるメカニズムを理解する.
主な方法:
- EBER遺伝子プロモーター領域の分析,内遺伝子制御領域 (A,Bボックス) と上流要素を含む.
- DNAase I足跡分析は,タンパク質とDNAの相互作用を特定するために行われます.
- オリゴヌクレオチドコンペティションアッセイでは,転写因子結合を確認します.
主要な成果:
- EBER遺伝子は,機能的なAとBボックス内遺伝子制御領域を有しています.
- 3つのアップストリーム要素が特定され,インビボ発現を50倍まで刺激しました.
- DNAase Iの足跡調査により,活性化トランスクリプション因子 (ATF) または関連するタンパク質が -40から -55.5までのヌクレオチドに結合することが明らかになった.
- Sp1タンパク質または関連するタンパク質が,核酸−56~−77.7に結合する.
- -23 と -28 の位置の間の要素は,TATA ボックスに似ていた.
結論:
- EBERは,異なるプロモーター要素によって調節される珍しいRNAポリメラーゼIIIのトランスクリプトです.
- ATFとSp1-のような転写因子は,EBER発現を制御する上で重要な役割を果たします.
- これらの調節メカニズムは,EBVが変異した細胞におけるEBERの豊富な存在に寄与している可能性が高い.
関連する概念動画
Bacterial RNA Polymerase
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In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
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All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
The Eukaryotic Promoter Region
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
Transcription Initiation
Initiation is the first step of transcription in eukaryotes. Prokaryotic RNA Polymerase (RNAP) can bind to the template DNA and start transcribing. On the other hand, transcription in eukaryotes requires additional proteins, called transcription factors, to first bind to the promoter region in the DNA template. This binding helps recruit the specific RNAP that can assemble on the DNA and start transcription.
The promoters and enhancers and their accessory proteins allow tight regulation of...
The promoters and enhancers and their accessory proteins allow tight regulation of...
Bacterial RNA Polymerase
Unlike eukaryotes, bacteria use a single RNA Polymerase (RNAP) to transcribe all genes. The different subunits of bacterial RNAPhave distinct functions. The multisubunit structure of the bacterial RNAP helps the enzyme to maintain catalytic function, facilitate assembly, interact with DNA and RNA, and self-regulate its activity.
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
In most genes, the transcription site is a single base present upstream of the coding sequence. Though RNAP is a catalytically efficient enzyme, it does not recognize...
Eukaryotic RNA Polymerases
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...


