ゲノムエディティング. CRISPR-Cas9によるゲノム工学の新たな境界線
Jennifer A Doudna1, Emmanuelle Charpentier2
1Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley, CA 94720, USA. Department of Chemistry, University of California, Berkeley, CA 94720, USA. Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA. doudna@berkeley.edu emmanuelle.charpentier@helmholtz-hzi.de.
Science (New York, N.Y.)
|November 29, 2014
まとめ
CRISPR-Cas9システムは,多様な生物のゲノム工学を容易に可能にし,生物学的研究とバイオテクノロジーを革命的に変えました. この強力なツールは,遺伝子改変を容易にし,発見と治療開発のための新しい道を開きます.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- バイオテクノロジー バイオテクノロジー
背景:
- CRISPR (クラスタリング・レギュラー・インタースペス・パリンドロミック・リピート) の生物学は,著しく進化した.
- 細菌のRNA誘導型CRISPR-Cas9システムは,ゲノム工学の重要なツールである.
研究 の 目的:
- CRISPR-Cas9.9の歴史とメカニズムを見直す.
- 生物学的研究とバイオテクノロジーに対するその変革的な影響を強調する.
主な方法:
- CRISPRの生物学の発見のレビュー.
- CRISPR-Cas9酵素メカニズムの解明.
主要な成果:
- CRISPR-Cas9は,生命領域のゲノム工学を容易にする.
- ゲノム操作は,もはや実験のボトルネックではありません.
結論:
- CRISPR-Cas9は,生物学的発見の新たな時代を告げています.
- 応用分野は,バイオテクノロジーとヒトの治療分野を網羅しています.
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