ミオゲン決定遺伝子のMyoD1のポジティブな自己調節
M J Thayer1, S J Tapscott, R L Davis
1Department of Genetics, Hutchinson Cancer Research Center, Seattle, Washington 98104.
Cell
|July 28, 1989
まとめ
MyoD1とミオゲニンは互いに活性化し,ポジティブなフィードバックループを形成します. この自己調節は,筋肉細胞のコミットメントを安定させ,ミオジェニックプログラムの活性化のための遺伝子発現を拡大するために不可欠です.
科学分野:
- 分子生物学は分子生物学である.
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
背景:
- MyoD1とミオゲニンは,筋肉の発達における重要な転写因子である.
- 特定の系統への細胞のコミットメントは,ミオゲン分化と同様に,安定した遺伝子発現プログラムを必要とします.
研究 の 目的:
- ミオゲニンの分化におけるMyoD1とミオゲニンの自己調節の可能性を調査する.
- MyoD1とミオゲニンの相互作用が,ミオゲニン結合の安定化における役割を明らかにする.
主な方法:
- 10T1/2細胞および他の細胞系をMyoD1およびmyogeninのためのcDNA発現ベクトルで変異させる.
- RT-PCRまたは同様の技術を使用して,内生的なMyoD1およびミオゲニンmRNA発現レベルの定量分析.
主要な成果:
- MyoD1cDNAによるトランスフェクションにより,10T1/2およびスイス3T6細胞における内生的なMyoD1mRNA発現が誘発され,ポジティブな自己調節が示された.
- MyoD1トランスフェクションはまた,ミオゲニン発現を活性化し,ミオゲニントランスフェクションはMyoD1発現を活性化しました.
- この自己調節は細胞型特異的であり,すべての試験された線維芽細胞またはアディポ芽細胞系では観察されなかった.
結論:
- MyoD1とミオゲニンは正の自己調節ループに参加します.
- このループは,菌根性コミットメントの安定に寄与する可能性がある.
- このループは,MyoD1とミオゲニンの発現を,ミオゲニンプログラムを活性化するために必要な値まで増幅する可能性があります.
さらに関連する動画
14:47Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry
Published on: May 17, 2016
11:02Identification and Analysis of Myogenic Progenitors In Vivo During Acute Skeletal Muscle Injury by High-Dimensional Single-Cell Mass Cytometry
Published on: December 1, 2023
関連する概念動画
Master Transcription Regulators
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
Abnormal Proliferation
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the daughter...
Formation of Muscle Fibers from Myoblasts
De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Satellite Stem Cells and Muscular Dystrophy
Satellite stem cells or myosatellite cells are quiescent stem cells that Alexander Mauro first identified in 1961. These cells are located between the sarcolemma, the plasma membrane of muscle fibers, and the basal lamina, the connective tissue sheath covering it. These mononucleated cells are activated in response to muscle injury, can transform into myoblasts, and may form or repair muscle fibers. Myosatellite cells can provide additional myonuclei for muscle regeneration or return to a...
Smooth Muscle Contraction
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
Autoregulation of Blood Flow
Autoregulation mechanisms are characterized by their inherent capacity for self-regulation without necessitating specific nervous stimulation or endocrine control. These mechanisms facilitate the adjustment of blood flow and, therefore, perfusion specific to each tissue region. This self-regulation encompasses chemical signals and myogenic controls.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
Chemical Signaling in Autoregulation
Chemical signaling operates at the precapillary sphincter level, inciting either contraction or relaxation.
