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恒常的なサイズ拡張は,細菌の細胞サイズホメオスタシスを駆動します

Manuel Campos¹, Ivan V Surovtsev¹, Setsu Kato²

¹Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA.

|December 7, 2014

まとめ

バクテリアは,細胞の大きさを限界によって制御するのではなく,分裂の間に一貫した量を増やすことによって制御します. この強固なメカニズムは,Escherichia coliとCaulobacter crescentusの細胞サイズホメオスタシスを確保する.

科学分野:

微生物学微生物学とは
細胞生物学細胞生物学
システム生物学システム生物学

背景:

細胞サイズのコントロールは,細胞サイクル進行において極めて重要です.
支配的なモデルは,細菌が重要なサイズ値で分裂することを示唆しました.

研究の目的:

細菌の細胞サイズ制御のための臨界サイズパラダイムを実験的にテストする.
進化的に遠い細菌における細胞サイズホメオスタシスのメカニズムを解明する.

主な方法:

単細胞顕微鏡を用いて,細菌の成長と分裂を追跡した.
細胞サイズダイナミクスを分析するために数学モデリングが使用されました.

主要な成果:

直接的な実験的証拠は,臨界サイズ値モデルを否定した.
Escherichia coliとCaulobacter crescentusは,細胞の初期サイズに関係なく,分裂の間の一定のサイズ増加によってサイズホメオスタシスを達成します.
このメカニズムは,数世代に渡って,大きさの変動を指数関数的に強烈に減少させます.

結論:

バクテリア細胞のサイズ制御は,臨界サイズではなく,恒常的な成長インクリメントメカニズムによって達成されます.
この原則は,さまざまな部門タイプと成長率に適用されます.
恒定サイズの拡張は,分裂過程の近くで制御される可能性があり,細胞サイクル制御に関する新しい洞察を提供します.

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