トウモロコシのSpmトランスポーザブルエレメントのエッセンシャル・ラッグ・トランスクリプトは,代替スプライシングによって生成されます
P Masson1, G Rutherford, J A Banks
1Department of Embryology Carnegie Institution of Washington Baltimore, Maryland 21210.
Cell
|August 25, 1989
まとめ
新しいサプレッサー-ミュータータ (Spm) 移植可能な要素遺伝子が特定されました. Spm遺伝子内のイントロン配列は,必須製品をコードし,新しい翻訳的調節に関与し,植物における遺伝子活動に影響を与えます.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 植物科学 植物科学について
背景:
- サプレッサー・ミュータータ (Spm) は,転置可能な要素のクラスである.
- Spm遺伝子の機能を理解することは,植物遺伝学にとって極めて重要です.
研究 の 目的:
- 新規のサプレッサー変異体 (Spm) 移植可能要素遺伝子を特定する.
- Spm遺伝子の活性と調節におけるイントロン配列の役割を調査する.
主な方法:
- In vitroミュータゲネシスによる
- cDNAクローニング
- トランスジェニックタバコの分析
主要な成果:
- tnpA遺伝子のイントロンのフレームシフト変異により,SPMの活性が著しく低下しました.
- エクソンとイントロンの配列を含む,代替的にスプライスされたトランスクリプト (tnpB, tnpC, tnpD) を特定した.
- エクソン1で2つの代替スプライスドナーサイトを発見した.
結論:
- Spmイントロン配列は,必須の遺伝子製品をコードする.
- Spmは,多様なトランスクリプトを生成するために,代替スプライシングを使用します.
- エクソン1の構造によって,変異調節の新しい方法が示唆されている.
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