Aδ-LTMRsの方向選択性の細胞および分子基礎
Michael Rutlin1, Cheng-Ying Ho2, Victoria E Abraira3
1Solomon H. Snyder Department of Neuroscience, Howard Hughes Medical Institute, The Johns Hopkins University School of Medicine, 725 North Wolfe Street, Baltimore, MD 21205, USA.
Cell
|December 20, 2014
まとめ
低値メカノセンサリーニューロン (LTMRs) は,毛の傾斜方向を検知する. BDNFシグナリングは,これらのLTMRの偏分を誘導し,指向的なタッチ知覚を可能にします.
科学分野:
- 神経科学は神経科学である.
- ソマティック・センサリーシステム
- メカノトランスデュークション
背景:
- 触覚の知覚は,皮膚にある低値メカニカセンサリーニューロン (LTMRs) に依存しています.
- LTMRは,皮膚の動きの方向を含む刺激を検出するために不可欠です.
研究 の 目的:
- ネズミのAδ-LTMRsによって媒介される方向選択的触覚の背後にあるメカニズムを調査する.
- BDNF-TrkBシグナル伝達が,Aδ-LTMRの極化およびその方向性チューニングにおける役割を決定する.
主な方法:
- ネズミのAδ-LTMRsの配分と応答特性を毛皮と関連して調べました.
- BDNFとその受容体TrkBの発現と局所化を,毛皮の表皮細胞とAδ-LTMRsで調査した.
- Aδ-LTMRの極化と機能への影響を評価するために,毛皮の表皮細胞におけるBDNFの遺伝的アブレーションを利用した.
主要な成果:
- ネズミのAδ-LTMRは,尾毛からロストラ毛の傾斜に好ましいチューニングを示しています.
- Aδ-LTMR ランセオラト結末は,毛の葉っぱの尾部側に集中し,極化しています.
- BDNFは,TrkBを発現するAδ-LTMR末端の近く,毛の尾側で合成されます.
- BDNFの消去は,Aδ-LTMRの偏分を妨害し,その応答方向をランダム化します.
結論:
- BDNF-TrkBシグナリングは,Aδ-LTMRランセオラート末端の極化に不可欠です.
- この指向的偏極化は,Aδ-LTMRsが髪の歪み方向を感知する能力を支えている.
- この発見は,指向的な触覚感覚のための分子機構を明らかにしています.
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