ホメオボックスを含む成長ホルモン特異的転写因子GHF-1の浄化
J L Castrillo1, M Bodner, M Karin
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
Science (New York, N.Y.)
|February 10, 1989
まとめ
成長ホルモン (GH) 遺伝子発現はGHF-1によって制御されます. しかし,GHF-1はプロラクチン (Prl) 遺伝子発現を調節しないため,これらの下垂体遺伝子を異なる要因が支配することを示しています.
科学分野:
- 分子内分泌学分子内分泌学
- 遺伝子規制 遺伝子規制
- タンパク質とDNAの相互作用
背景:
- 下垂体特異的な遺伝子発現は,ホルモン産生に不可欠である.
- 成長ホルモン (GH) とプロラクチン (Prl) は,下垂体ホルモンの主要ホルモンである.
- ホメオボックスの転写因子であるGHF-1は,GH遺伝子発現を調節する.
研究 の 目的:
- GHF-1がプロラクチン (Prl) 遺伝子発現を制御するかどうかを調査する.
- pituitary-specific遺伝子調節に関与するトランス作用因子を特定する.
主な方法:
- 下垂体腫瘍細胞抽出物からGHF-1の浄化.
- エレクトロフォレティックモビリティシフトアッセイでは,プロモーター結合を評価する.
- GHF-1に対する抗体を用いたウエスタン・ブロット分析.
主要な成果:
- GHF-1 (33 kDa) は,GHプロモーターを特定結合して活性化しますが,Prlプロモーターは活性化していません.
- Prl プロモーターに結合したGHF-1から分離可能な,別々の因子である.
- GHF-1抗体は,Prl結合活性とクロス反応しなかった.
結論:
- GHとPrlの垂体特異的発現は,異なるトランス作用因子によって調節されます.
- GHF-1はGH遺伝子の調節に特異的です.
- Prl遺伝子発現を制御する別の要因があります.
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