ミトコンドリアアセンブリファクタをコードする酵母HSP60遺伝子の特徴づけ
D S Reading1, R L Hallberg, A M Myers
1Department of Zoology, Iowa State University, Ames 50011.
Nature
|February 16, 1989
まとめ
イーストの熱ショックタンパク質60 (hsp60) は,HSP60遺伝子によってコードされています. このタンパク質は,ミトコンドリア内のタンパク質複合体の組み立てを支援し,タンパク質の折りたたみにおける保存された役割を示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 細胞生物学 細胞生物学
- 遺伝学 遺伝学とは
背景:
- 熱ショックタンパク質 (hsp60) は種間で保存され,ストレス反応に関与しています.
- hsp60の同種は,細菌 (例えば,Escherichia coli groEL) と有機体 (例えば,植物クロロプラスト,動物ミトコンドリア) に見られます.
研究 の 目的:
- 酵母 (Saccharomyces cerevisiae) のhsp60ホモログをコードする核遺伝子 (HSP60) をクローンし,特徴づけること.
- 細胞プロセス,特にミトコンドリア複合体の組み立てにおける酵母hsp60の機能を調査する.
主な方法:
- 酵母HSP60遺伝子の遺伝子クローンと核酸配列解析.
- HSP60遺伝子の役割の特徴付けは,以前に特定された条件付き-致死性変異を用いて行われました.
主要な成果:
- 酵母HSP60遺伝子はクローンされ,配列化され,E. coli groELとクロロプラストRUBISCO結合タンパク質 (RBP) との同質性を明らかにしました.
- HSP60遺伝子は,ミトコンドリアのマルチメリック複合体組成の欠陥を引き起こす突然変異の場所として特定されました.
結論:
- 酵母hsp60は,細菌のGroELと植物/動物のミトコンドリアチャペロンに同型である.
- GroELに関連するタンパク質は,タンパク質の折りたたみおよび/または複数のサブユニット複合体の組み立てを促進する進化的に保存された機能を果たしている可能性が高い.
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