ネズミのT細胞前駆体の特徴は,チモトキシンへの化学的移住によって並べられています
M A Deugnier1, B A Imhof, B Bauvois
1Laboratoire de Physiopathologie du Développement CNRS et Ecole Normale Supérieure, Paris, France.
Cell
|March 24, 1989
まとめ
ネズミのチム細胞系は,骨髄から未成熟のT細胞前体を引き付けるペプチドであるチモトキシンを分泌する. この化学反応のメカニズムは,早期のT細胞発達の研究のために,T細胞前細胞を分離することができます.
科学分野:
- 免疫学 免疫学とは
- 発達生物学 発達生物学とは
- 細胞生物学 細胞生物学
背景:
- 甲状腺はT細胞の成熟に不可欠です.
- 早期のT細胞発達の理解には,前駆体細胞を特定し,隔離することが必要です.
- ケモタキシスは,発達中の細胞移動に作用する.
研究 の 目的:
- T細胞前駆体を引き付ける要素を特定する.
- ティモトキシン反応性細胞のフェノタイプと行動を特徴付ける.
- T前細胞を隔離するキモタキシスの可能性を評価する.
主な方法:
- ネズミの甲状腺細胞系を培養する.
- 分泌されたペプチド (チモトキシン) の測定.
- 青年ラットの骨髄細胞を用いたケモタキシスアッセイ.
- 細胞フェノタイプ (Thy-1) を分析するためのフローサイトメトリ.
- 胸膜ストロマとの共培養.
- T細胞受容体 (TCR) 遺伝子発現の分析.
主要な成果:
- 甲状腺の細胞系は,チモトキシン (11 kdペプチド) を分泌する.
- ティモタキシンは,骨髄から低密度で複製しないリンパ性細胞の希少なサブセット (0.1%) を引き寄せます.
- これらの細胞は未成熟のThy-1+T-B-現象型を持っています.
- 細胞は生存とT細胞の微分化のために胸膜ストロマを必要とします.
- 排列された細胞は,TCRアルファとベータ鎖のトランスクリプトを表現し,T細胞のコミットメントを示します.
結論:
- ティモトキシン (Thymotaxin) は,T細胞前細胞の化学誘致剤である.
- 化学的分類は,骨髄からT細胞前駆体を分離する有効な方法である.
- 排序された細胞は,リンパ系にコミットした休息している幹細胞です.
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