狭い交差点. 狭い交差点. 狭い交差点. 狭い交差点. 狭い交差点. 狭い交差点. 狭い交差点. 狭い交差点. Clostridium perfringens enterotoxinによる緊密な交差点の解体に関する構造的な洞察
Yasunori Saitoh1, Hiroshi Suzuki2, Kazutoshi Tani2
1Cellular and Structural Physiology Institute, Nagoya University, Chikusa, Nagoya 464-8601, Japan. Department of Basic Medical Science, Graduate School of Pharmaceutical Science, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
まとめ
クロストリジウム・パーフリンゲンスの腸内毒素 (C-CPE) は,クラウジン-19に結合し,緊密な接点を破壊する. 構造分析は,C-CPEの相互作用がキーヘリクスを移動させ,TJの解体と透過性の増加を説明することを明らかにします.
科学分野:
- 分子生物学は分子生物学である.
- 構造生物学 構造生物学とは
- 細胞生物学 細胞生物学
背景:
- クロストリジウム・パーフリンゲンスの腸内毒素 (C-CPE) はクラウディンを標的とし,緊密な結節 (TJ) の解体と上皮の浸透性の増加につながります.
- C-CPEとクラウジンの間の分子相互作用を理解することは,C-CPEの病原性メカニズムを解明するために重要です.
研究 の 目的:
- 哺乳類のクラウジン-19とC-CPE.の間で形成された複合体の高解像度構造を決定する.
- 緊密な接合点のC-CPE媒介による破壊の構造的基礎を解明する.
主な方法:
- X線結晶学を用いて,クラウジン-19/C-CPE複合体の構造を3.7 Å解像度で得られた.
主要な成果:
- 構造は,C-CPEとクラウジン-19の細胞外セグメントの間の広範囲な水性および水性相互作用を明らかにします.
- クラウジンのTJ鎖の組み立てに不可欠な重要な細胞外ヘリックスが,クラウジン-19/C-CPE複合体の構造に欠けていた.
- この欠落は,C-CPE結合がこのヘリクスを移動し,TJ解体につながる可能性があることを示唆しています.
結論:
- C-CPEのC末端領域は,クラウジン-19と相互作用し,TJ構造を破壊する.
- C-CPE結合による臨界細胞外ヘリクスの移位は,C-CPE誘発の緊密な接合の解体とパラセルラー透過性の増加のための可能性が高いメカニズムです.
さらに関連する動画
関連する概念動画
Tight Junctions
9.0K
Tight junctions are molecular seals between cells that prevent the leaking of fluids, ions, and other small solutes across cavities and compartments in multicellular organisms. They are mainly composed of claudin and occludin transmembrane proteins, and other proteins such as tricellulin and JAM (junctional adhesion molecule). All these proteins are 4-pass transmembrane proteins, except JAM, which is a single-pass transmembrane protein belonging to the immunoglobulin superfamily. The...
9.0K
Adherens Junctions
7.9K
Strong contact points between adjacent cells anchor them to each other, forming tissues. Such anchoring junctions are of two types – adherens junctions and desmosomes. Adherens junctions are abundant in tissues such as epithelium and endothelium, forming a continuous zone of adhesion called the adhesion belt. In other tissues, such as heart muscle, they appear as clusters, linking the cells to produce coordinated heart muscle contraction.
Adherens Junctions are Dynamic
Adherens Junctions are Dynamic
7.9K
Tension Response at Adherens Junctions
4.3K
The adherens junctions that anchor cells together are multi-protein complexes that dynamically adapt to mechanical stimuli such as tensile forces and shear stress. Mechanosensory proteins in these junctions can sense such mechanical stimuli and undergo a shift in their conformation, resulting in an altered function — a process called mechanotransduction.
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
α-Catenin as a Mechanosensory Protein
The α-catenin of adherens junctions is an allosteric protein with three VH (vinculin...
4.3K
Overview of Cell-Cell Junctions
32.4K
The complex three-dimensional arrangement of cells in any multicellular organism is defined and maintained by interactions of cells with each other and the extracellular matrix. Cell-cell junctions are specialized structures where the multi-protein complexes on one cell interact with the multi-protein complexes on another cell. These cell junctions are classified into three main types based on their function — occluding, anchoring, and gap junctions.
Occluding or Tight...
Occluding or Tight...
32.4K
Overview of Cell-Cell Junctions
34.3K
34.3K
Bacterial Toxins
108
Bacterial toxins are sophisticated virulence factors that enable pathogenic bacteria to interact with, invade, and damage host tissues. These toxins fall broadly into two types: protein exotoxins, which are secreted into the environment and target specific host receptors, and lipopolysaccharide endotoxins, which are structural components of the bacterial outer membrane released primarily during bacterial lysis or membrane shedding. Exotoxins generally act more selectively, binding to cell...
108


