関連する実験動画
Updated: Aug 13, 2026
12:13
Zinc-finger Nuclease Enhanced Gene Targeting in Human Embryonic Stem Cells
Published on: August 23, 2014
pituitary 特定の転写因子 GHF-1 の機能ドメインの解剖
L E Theill1, J L Castrillo, D Wu
1Department of Pharmacology, School of Medicine, University of California, San Diego, La Jolla 92093.
Nature
|December 21, 1989
まとめ
pituitary細胞における成長ホルモン (GH) 遺伝子発現は,GHF-1転写因子に依存しています. GHF-1ホメオドメインはDNAを結合し,そのPOU特異ドメインは細胞特異の遺伝子調節のための活性を強化します.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- エンドクリノロジー エンドクリノロジー
背景:
- 前垂体体内のソマトトロフィック細胞における成長ホルモン (GH) の特異的発現は,GH遺伝子の5'側面領域のプロモーターによって制御されます.
- このプロモーターには,マウスの下垂体発達におけるGH遺伝子活性化に不可欠な転写因子GHF-1の結合部位が含まれています.
研究 の 目的:
- 配列特異的なDNA結合と転写活性化に関与するGHF-1転写因子の機能領域を調査する.
- GH遺伝子の細胞型特異的発現におけるGHF-1の役割を明らかにする.
主な方法:
- DNA結合と転写活性化に関与するGHF-1タンパク質ドメインの分析.
- GHF-1ドメインとGH遺伝子プロモーターの相互作用に関する研究.
主要な成果:
- GHF-1ホメオドメインは,配列特異のDNA結合に十分である.
- GHF-1のPOU固有のドメインはDNA結合活動を刺激しますが,DNAと直接相互作用しません.
- 転写活性化は,ヒドロキシル化されたアミノ酸に富んだ独特のドメインによって媒介されます.
結論:
- GHF-1は,GH遺伝子の細胞型特異的発現の重要な決定因子である.
- GHF-1の異なるドメインは,DNA結合と転写調節において特定の役割を果たします.
- GHF-1の構造モチーフに似た構造モチーフは,他の細胞型特異の転写因子にも見られます.
関連する概念動画
02:33Hedgehog Signaling Pathway
The Hedgehog gene (Hh) was first discovered due to its control of the growth of disorganized, hair-like bristles phenotype in Drosophila, much like hedgehog spines. Hh plays a crucial role in the development of organs and the maintenance of homeostasis in both invertebrates and vertebrates. However, while Drosophila has only one Hh protein, mammals have multiple functional Hedgehog proteins - Sonic (Shh), Desert (Dhh), and Indian Hedgehog (Ihh). All of these homologous proteins have adapted to...
01:16TGF - β Signaling Pathway
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors are of three kinds RI, RII, and RIII. The RI...
01:17The Pituitary Gland
The pituitary is a small endocrine organ in the sphenoid bone under the hypothalamus. Primarily, the pituitary in adults has two distinct anatomical and functional regions— the anterior and posterior lobes. During human fetal development, a third pituitary gland region called the pars intermedia atrophies and disappears. However, some of its cells migrate and exist adjacent to the anterior pituitary in adults.
01:27Hormones of the Pituitary Gland
The small, pea-sized pituitary gland is located at the base of the brain. It is crucial in regulating various bodily functions, from growth to reproduction. The gland is divided into the anterior lobe and the posterior lobe. The secretory cell clusters in the pars distalis of the anterior pituitary lobe are controlled by hypothalamic regulators and synthesize six primary hormones.
The most abundantly secreted hormone from the anterior lobe is the growth hormone, which controls overall growth by...
The most abundantly secreted hormone from the anterior lobe is the growth hormone, which controls overall growth by...