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関連する概念動画

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

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Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
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MALDI-TOF Mass Spectrometry01:19

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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.Matrix-assisted laser desorption ionization (MALDI) is a commonly...
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Mass Spectrometers01:16

Mass Spectrometers

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This lesson details the instrumentation of a mass spectrometer—a physical instrument to perform mass spectrometry on analyte molecules and record the characteristic mass spectra. This is achieved via three chief functions:
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Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

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In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then...
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Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

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An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a soft-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.To...
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Chemical Ionization (CI) Mass Spectrometry01:21

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The molecular ion peak of a molecule in the mass spectrum provides vital information for molecular identification. However, conventional electron impact ionization can lead to the rapid dissociation of some molecular ions before they reach the detector. A milder ionization method is required to increase the lifetime of such ionized analyte molecules. Chemical ionization (CI) is a gas-phase protonation reaction useful for mass-analyzing analyte molecules that are easily protonated to yield the...
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Atmospheric-pressure Molecular Imaging of Biological Tissues and Biofilms by LAESI Mass Spectrometry
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レーザー誘発のポストイオニゼーションによる質量スペクトロメトリ画像撮影.

Jens Soltwisch1, Hans Kettling2, Simeon Vens-Cappell2

  • 1Institute for Hygiene, University of Münster, Robert-Koch-Strasse 41, 48149 Münster, Germany.

Science (New York, N.Y.)
|March 7, 2015
PubMed
まとめ

この研究では,マトリックスアシストレーザー脱吸収/イオン化質量スペクトロメトリ画像 (MALDI-MSI) の感度を大幅に高めるための二次イオン化技術が導入されています. この方法は,組織内の様々なバイオ分子検出を向上させ,イメージング能力を向上させます.

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Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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Imaging Metals in Brain Tissue by Laser Ablation - Inductively Coupled Plasma - Mass Spectrometry LA-ICP-MS
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Whole-body Mass Spectrometry Imaging by Infrared Matrix-assisted Laser Desorption Electrospray Ionization IR-MALDESI
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科学分野:

  • アナリティカル・ケミストリー (Analytical Chemistry) とは
  • バイオテクノロジー バイオテクノロジー
  • 質量スペクトロメトリー画像処理

背景:

  • マトリックスアシストレーザー脱吸収/イオン化質量スペクトロメトリ画像 (MALDI-MSI) は,組織内のバイオ分子をマッピングするための強力なツールです.
  • 現在のMALDI-MSI方法は,限られたイオン化の効率により,感度が制限されています.
  • 低イオン化率は,多様なバイオ分子クラスの包括的な分析を妨げます.

研究 の 目的:

  • MALDI-MSI.でイオン出力を高めるための新しいポストイオン化方法を開発する.
  • 脂質,ビタミン,サッカライドに対する感度と検出能力を向上させる.
  • 感度が向上した高側面解像度イメージングを実現するために.

主な方法:

  • 波長調整可能なポストイオン化レーザーを使って,ガス相における二次的なMALDIのようなイオン化を誘導した.
  • 動物および植物組織サンプルを分析するためにこの技術を適用しました.
  • 冷却ガス圧力,レーザー波長,パルスエネルギー,パルス間遅延など,調査された重要なパラメータ.

主要な成果:

  • 脂質,脂溶性ビタミン,サッカライドのイオン収量で最大2度の増加を達成した.
  • 5マイクロメートルのレーザースポットサイズで画像撮影が成功したことを実証しました.
  • 二次性イオン化のプロセスを最適化するために重要なキーパラメータを特定しました.

結論:

  • 開発されたポストイオン化レーザー技術は,MALDI-MSIの感受性を大幅に高めています.
  • この進歩により,組織における生物分子分布の分析がより包括的に可能になる.
  • この方法は,低マイクロメートルの横断解像度で敏感なMALDI-MSイメージングの可能性を秘めています.