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Replication in Eukaryotes01:29

Replication in Eukaryotes

19.5K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
19.5K
Replication in Eukaryotes02:31

Replication in Eukaryotes

208.6K
Overview
208.6K
The Replisome03:01

The Replisome

39.9K
DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
The synthesis of the leading and lagging strands is a highly coordinated process. To explain this, the “Trombone model” was proposed by Bruce Alberts in 1980. The DNA loop formation starts when a primer is synthesized on the parent lagging strand. The loop grows with...
39.9K
Chromosome Structure02:40

Chromosome Structure

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A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
28.1K
Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
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Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

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関連する実験動画

Updated: Apr 16, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

10.5K

ユカリオット起源認識複合体の結晶構造

Franziska Bleichert1, Michael R Botchan2, James M Berger1

  • 1Department of Biophysics and Biophysical Chemistry, Johns Hopkins School of Medicine, Baltimore, Maryland 21205, USA.

Nature
|March 13, 2015
PubMed
まとめ
この要約は機械生成です。

起源認識複合体 (ORC) 構造は,DNA複製の開始を制御するための新しいメカニズムを明らかにします. この構造は,ORCが活性状態と抑制状態の間で切り替えることができ,細胞サイクル調節に影響を及ぼすことを示唆しています.

さらに関連する動画

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
13:02

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Published on: December 28, 2019

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Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

10.4K

関連する実験動画

Last Updated: Apr 16, 2026

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
09:15

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

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Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO
13:02

Production, Crystallization, and Structure Determination of the IKK-binding Domain of NEMO

Published on: December 28, 2019

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Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes
11:42

Iterative Optimization of DNA Duplexes for Crystallization of SeqA-DNA Complexes

Published on: November 1, 2012

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科学分野:

  • 構造生物学 構造生物学とは
  • 分子生物学は分子生物学である.
  • 細胞サイクル調節 細胞サイクル調節

背景:

  • 細胞DNA複製の開始は,ゲノムの安定性にとって極めて重要です.
  • ヘテロヘクスアメリカン起源認識複合体 (ORC) は,真核生物における複製の開始を指揮する.

研究 の 目的:

  • ドロソフィラ ORCの結晶構造を決定するために.
  • DNA複製の開始におけるORC機能の構造的基礎を解明する.

主な方法:

  • 3.5 Åの解像度のX線結晶学.
  • 270 kDa の ORC コア 複合体の構造分析.

主要な成果:

  • ORC構造は,翼状ヘリックスとAAA+ドメインを持つ2層のリングを明らかにします.
  • 予期せぬドメイン間の交換と準螺旋的なDNA結合チャネルが観察されました.
  • Orc1 AAA+ドメインの有意な回転は,自己抑制のメカニズムを示唆しています.

結論:

  • ドロソフィラ・ORCはDNAを囲み,ヘリカーゼ負荷のためのMCM2-7複合体を誘導する.
  • 構造は,ORCが活性型と自己抑制型との間の移行が可能であることを示しています.
  • このコンフォーマーションスイッチングは,細胞サイクル制御のための新しい規制メカニズムを提供します.