関連する実験動画

Updated: Mar 7, 2026

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research
09:53

Author Spotlight: Advancing Techniques and Discoveries in Protein Synthesis and Assembly Through Innovative Mitochondrial Research

Published on: June 7, 2024

1.7K

イーストミトコンドリアのリボソームおよびメッセンジャーRNA前駆体の自己スプライシング

G van der Horst, H F Tabak

    Cell
    |April 1, 1985
    PubMed
    まとめ

    酵母菌のミトコンドリア前駆体RNAは,タンパク質なしで自己スプライシングを in vitro で行います. このプロセスは,GTPの組み込み,エクソン結合,およびイントロンの循環化を含み,RNAを証明します.

    さらに関連する動画

    ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast07:31

    ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

    Published on: June 30, 2022

    3.0K
    Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation14:44

    Isolation of mRNAs Associated with Yeast Mitochondria to Study Mechanisms of Localized Translation

    Published on: March 14, 2014

    13.4K

    関連する概念動画

    RNA Splicing01:32

    RNA Splicing

    Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
    61.0K
    Ribosomal RNA Synthesis02:53

    Ribosomal RNA Synthesis

    Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
    Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
    15.0K
    Ribosomal RNA Synthesis02:53

    Ribosomal RNA Synthesis

    No description available
    4.7K
    Mitochondrial Precursor Proteins01:39

    Mitochondrial Precursor Proteins

    Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70  chaperones are targetted to TOM20-TOM22 receptor complexes.
    Most of the mitochondrial...
    3.9K
    pre-mRNA Processing02:01

    pre-mRNA Processing

    In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
    Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl...
    57.8K
    Alternative RNA Splicing02:18

    Alternative RNA Splicing

    Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
    There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
    25.5K