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抗原的変異は,再発性ボルレリア熱におけるDNAの再編成と関連しています

J T Meier, M I Simon, A G Barbour

|June 1, 1985

まとめ

発熱再発の原因であるBorrelia hermsiiは,免疫システムを回避するために表面タンパク質を変更します. この研究では,これらの変化にはDNAの再編成と遺伝子発現が含まれていることが示されています.

科学分野:

微生物学微生物学とは
免疫学免疫学とは
遺伝学遺伝学とは

背景:

Borrelia hermsiiは,抗原変異によって再発性発熱を引き起こす.
表面タンパク質は,Borrelia hermsiiの血清型を決定する.
抗原的変異は,病原体が宿主の免疫反応を回避することを可能にします.

研究の目的:

Borrelia hermsii.の抗原変異の基礎となる分子機構を調査する.
異なる表面タンパク質の発現にDNAの再編成が関与しているかどうかを判断する.

主な方法:

変数表面タンパク質のアミノ酸配列を基に合成された混合配列オリゴヌクレオチド.
北部と南部のブロッティングを用いて3つの同位性のB. hermsiiのmRNAとDNAを検出した.
更に分析するために,血清型特異のDNA断片をクローンした.

主要な成果:

オリゴヌクレオチドプローブは,mRNAへの血清型特異的ハイブリダイゼーションを示した.
南部の斑点は,一般的なDNA断片と,血清型特異的なDNA断片の両方を明らかにしました.
クローンされた血清型特異のDNA断片は,DNAの再編成との関連性を確認した.

結論:

Borrelia hermsiiの抗原変異は,DNAの再編成に関連しています.
抗原特異遺伝子の発現関連コピーは,血清型の切り替えに寄与する可能性が高い.
このメカニズムは,再発する熱性感染症の際に免疫逃避を可能にします.

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